Oligodendrocyte precursor differentiation is perturbed in the absence of the cyclin-dependent kinase inhibitor p27(Kip1) Journal Article
| Authors: | Casaccia-Bonnefil, P.; Tikoo, R.; Kiyokawa, H.; Friedrich Jr, V.; Chao, M. V.; Koff, A. |
| Article Title: | Oligodendrocyte precursor differentiation is perturbed in the absence of the cyclin-dependent kinase inhibitor p27(Kip1) |
| Abstract: | During development of the central nervous system, oligodendrocyte progenitor cells (O-2A) undergo an orderly pattern of cell proliferation and differentiation, culminating in the ability of oligodendrocytes to myelinate axons. Here we report that p27(Kip1), a cyclin-dependent kinase inhibitor, is an important component of the decision of O-2A cells to withdraw from the cell cycle. In vitro, accumulation of p27 correlates with differentiation of oligodendrocytes. Furthermore, only a fraction of O-2A cells derived from p27-knockout mice differentiate successfully compared to controls. Inability to differentiate correlates with continued proliferation, suggesting that p27 is an important component of the machinery required for the G1/G0 transition in O-2A cells. In vivo, expansion of O-2A precursors before withdrawal, in part, leads to a greater number of oligodendrocytes. Together these data indicate a role for p27 during the decision to withdraw from the cell cycle in the oligodendrocyte lineage. |
| Keywords: | controlled study; nonhuman; cell proliferation; cell cycle protein; animal cell; mouse; animals; cell cycle proteins; mice; mice, knockout; animal tissue; cells, cultured; cell cycle; cell division; cell differentiation; animalia; stem cell; gene expression regulation; cyclin-dependent kinase inhibitor p27; tumor suppressor proteins; rat; glia cell; cyclin dependent kinase inhibitor; nerve cell differentiation; genes, tumor suppressor; myelination; differentiation; oligodendroglia; microtubule-associated proteins; priority journal; article; cki p27(kip1); oligodendrocyte progenitor cells |
| Journal Title: | Genes and Development |
| Volume: | 11 |
| Issue: | 18 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 1997-09-15 |
| Start Page: | 2335 |
| End Page: | 2346 |
| Language: | English |
| PUBMED: | 9308962 |
| PROVIDER: | scopus |
| PMCID: | PMC316517 |
| DOI: | 10.1101/gad.11.18.2335 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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