Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter Journal Article
| Authors: | Tolner, B.; Roy, K.; Sirotnak, F. M. |
| Article Title: | Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter |
| Abstract: | The structural organization of the murine RFC-1 gene encoding a folate transporter has been determined. The entire nucleotide sequence of the L1210 cell RFC-1 cDNA, the 3'- and 5'-untranslated regions and the coding sequence were found to be distributed in eight exons, including six primary exons and alternates to exon 1 and exon 5, spanning 10.4 kb. Splice variants were identified in an L1210 cell cDNA library. The most common incorporates exons 1 through 6, encoding a 58-kDa polypeptide. The two least common incorporate exons 1 and 2, a truncated version of exon 3 and exons 4 through 6; or exons 1 through 4, an alternate to exon 5, and exon 6, encoding polypeptides of 53.6 and 43.4 kDa, respectively. A fourth variant reported earlier (GenBank/EMBL accession No. L36539) by others incorporates what we have found to be an alternate of exon 1 and exons 2 through 6. A relatively GC-rich region of the genome just 5' of exon 1 as well as exon 1a appears to be distinctly promoter-like and encodes a number of putative cis-acting elements. The findings pertaining to alternates of exon 1 suggest that the transcription of RFC-1 variants results from two different promoters. |
| Keywords: | carrier protein; promoter region; sequence analysis; exon; exons; nonhuman; mouse; animals; mice; membrane proteins; gene library; cloning, molecular; molecular sequence data; sequence alignment; nucleotide sequence; carrier proteins; alternative splicing; alternative rna splicing; base sequence; folic acid; protein variant; biological transport; cis acting element; complementary dna; gene structure; membrane transport proteins; genetic organization; restriction mapping; promoter regions (genetics); priority journal; article; folate transport; murine rfc-1 gene; deoxyribonuclease ecori |
| Journal Title: | Gene |
| Volume: | 189 |
| Issue: | 1 |
| ISSN: | 0378-1119 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1997-04-11 |
| Start Page: | 1 |
| End Page: | 7 |
| Language: | English |
| DOI: | 10.1016/s0378-1119(96)00676-2 |
| PUBMED: | 9161403 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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