Registration of planar bioluminescence to magnetic resonance and x-ray computed tomography images as a platform for the development of bioluminescence tomography reconstruction algorithms Journal Article
| Authors: | Beattie, B. J.; Klose, A. D.; Le, C. H.; Longo, V. A.; Dobrenkov, K.; Vider, J.; Koutcher, J. A.; Blasberg, R. G. |
| Article Title: | Registration of planar bioluminescence to magnetic resonance and x-ray computed tomography images as a platform for the development of bioluminescence tomography reconstruction algorithms |
| Abstract: | The procedures we propose make possible the mapping of two-dimensional (2-D) bioluminescence image (BLI) data onto a skin surface derived from a three-dimensional (3-D) anatomical modality [magnetic resonance (MR) or computed tomography (CT)] dataset. This mapping allows anatomical information to be incorporated into bioluminescence tomography (BLT) reconstruction procedures and, when applied using sources visible to both optical and anatomical modalities, can be used to evaluate the accuracy of those reconstructions. Our procedures, based on immobilization of the animal and a priori determined fixed projective transforms, should be more robust and accurate than previously described efforts, which rely on a poorly constrained retrospectively determined warping of the 3-D anatomical information. Experiments conducted to measure the accuracy of the proposed registration procedure found it to have a mean error of 0.36+/-0.23 mm. Additional experiments highlight some of the confounds that are often overlooked in the BLT reconstruction process, and for two of these confounds, simple corrections are proposed. |
| Keywords: | nuclear magnetic resonance imaging; methodology; magnetic resonance imaging; sensitivity and specificity; reproducibility; reproducibility of results; mouse; animal; animals; mice; computer assisted tomography; image interpretation, computer-assisted; tomography, x-ray computed; algorithms; animal disease; algorithm; computer assisted diagnosis; evaluation; image enhancement; image subtraction; instrumentation; luminescence; optical tomography; whole body imaging; luminescent measurements; subtraction technique; tomography, optical |
| Journal Title: | Journal of Biomedical Optics |
| Volume: | 14 |
| Issue: | 2 |
| ISSN: | 1083-3668 |
| Publisher: | SPIE |
| Date Published: | 2009-03-01 |
| Start Page: | 024045 |
| Language: | English |
| DOI: | 10.1117/1.3120495 |
| PUBMED: | 19405773 |
| PROVIDER: | scopus |
| PMCID: | PMC2917449 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 30 November 2010" - "Source: Scopus" |
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