Rapid molecular detection and differentiation of influenza viruses A and B Journal Article
| Authors: | Otto, C. C.; Kaplan, S. E.; Stiles, J.; Mikhlina, A.; Lee, C.; Babady, N. E.; Tang, Y. W. |
| Article Title: | Rapid molecular detection and differentiation of influenza viruses A and B |
| Abstract: | Influenza is a contagious respiratory illness caused by influenza viruses A and B in humans and causes a significant amount of morbidity and mortality every year. The Influenza A and B assay was the first CLIA-waived molecular rapid flu test available. The Influenza A and B test works by employing isothermal amplification with influenza-specific primers followed by target detection with molecular beacon probes. Here, the performance of the Influenza A and B assay on frozen, archived nasopharyngeal swab (NPS) specimens stored in viral transport medium (VTM) were compared to a respiratory panel assay. The performance of the Influenza A and B assay was evaluated by comparing the results to the respiratory panel reference method. The sensitivity for total influenza virus A was 67.5% (95% CI (CI), 56.6-78.5) and the specificity was 86.9% (CI, 71.0-100). For influenza virus B testing, the sensitivity and specificity were 90.2% (CI, 68.5-100) and 98.8% (CI, 68.5-100), respectively. This system has the advantage of a significantly shorter test time than any other currently available molecular assay and the simple, pipette-free procedure runs on a fully integrated, closed, small-footprint system. Overall, the Influenza A and B assay evaluated in this study has the potential to serve as a point-of-care rapid influenza diagnostic test. © 2017 Journal of Visualized Experiments. |
| Keywords: | infection; influenza; molecular diagnostics; point-of-care testing; flu; influenza a and b assay; issue 119; respiratory specimens |
| Journal Title: | Journal of Visualized Experiments |
| Issue: | 119 |
| ISSN: | 1940-087X |
| Publisher: | MYJoVE Corporation |
| Date Published: | 2017-01-30 |
| Start Page: | e54312 |
| Language: | English |
| DOI: | 10.3791/54312 |
| PROVIDER: | scopus |
| PUBMED: | 28190065 |
| PMCID: | PMC5352307 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 March 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work