Detection of thyroglobulin, thyroid peroxidase, and RET/PTC1 mRNA transcripts in the peripheral blood of patients with thyroid disease Journal Article

  

Authors:	Tallini, G.; Ghossein, R. A.; Emanuel, J.; Gill, J.; Kinder, B.; Dimich, A. B.; Costa, J.; Robbins, R.; Burrow, G. N.; Rosai, J.
Article Title:	Detection of thyroglobulin, thyroid peroxidase, and RET/PTC1 mRNA transcripts in the peripheral blood of patients with thyroid disease
Abstract:	Purpose: Detection of mRNA transcripts for thyroglobulin (TG), thyroid peroxidase (TPO) and RET/PTC1 in the peripheral blood of patients with thyroid disease. Patients and Methods: TG, TPO, and RET/PTC1 mRNA were analyzed in 52 peripheral-blood samples from 44 patients diagnosed with thyroid carcinoma (24 patients), adenoma (five patients), and nodular hyperplasia (15 patients) by reverse transcription-polymerase chain reaction (RT-PCR). Results: TG and TPO were identified in 13 patients (54.2%) with thyroid carcinoma, which includes five of eight patients with no clinical evidence of disease at the time of blood collection. Four of 5 patients had cervical lymph node metastases and/or extrathyroid extension at the rime of the initial surgery. RET/PTC1 mRNA was detected in the peripheral blood of only one patient with papillary thyroid carcinoma, This sample was also positive for TG and TPO. TG and TPO were detected in two patients (10%) with benign thyroid nodules, All positive samples from patients with benign thyroid lesions were collected before surgery, whereas all samples collected after surgery were negative, RET/PTC1 mRNA was not detected in any of the patients with benign thyroid nodules, RT-PCR positivity for TG and TPO mRNA was higher in patients with carcinoma than in patients with benign lesions (P = .002). Conclusion: TG, TPO, and RET/PTC1 mRNA are detectable in the peripheral blood of patients with thyroid disease, which correlates with a diagnosis of carcinoma. (C) 1998 by American Society of Clinical Oncology.
Keywords:	detection; papillary carcinoma; prostate-cancer; molecular; messenger-rna; characterization; polymerase chain-reaction; circulating tumor-cells; reverse-transcriptase; neuroblastoma-cells; sensitive; ret oncogene
Journal Title:	Journal of Clinical Oncology
Volume:	16
Issue:	3
ISSN:	0732-183X
Publisher:	American Society of Clinical Oncology
Date Published:	1998-03-01
Start Page:	1158
End Page:	1166
Language:	English
ACCESSION:	WOS:000072258900043
PROVIDER:	wos
PUBMED:	9508203
DOI:	10.1200/JCO.1998.16.3.1158
Notes:	Article -- Source: Wos

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