Chromatin constrains the initiation and elongation of DNA replication Journal Article

   


Authors: Devbhandari, S.; Jiang, J.; Kumar, C.; Whitehouse, I.; Remus, D.
Article Title: Chromatin constrains the initiation and elongation of DNA replication
Abstract: Eukaryotic chromosomal DNA is faithfully replicated in a complex series of cell-cycle-regulated events that are incompletely understood. Here we report the reconstitution of DNA replication free in solution with purified proteins from the budding yeast Saccharomyces cerevisiae. The system recapitulates regulated bidirectional origin activation; synthesis of leading and lagging strands by the three replicative DNA polymerases Pol α, Pol δ, and Pol ε and canonical maturation of Okazaki fragments into continuous daughter strands. We uncover a dual regulatory role for chromatin during DNA replication: promoting origin dependence and determining Okazaki fragment length by restricting Pol δ progression. This system thus provides a functional platform for the detailed mechanistic analysis of eukaryotic chromosome replication. © 2017 Elsevier Inc.
Keywords: dna polymerase; dna replication; chromatin; okazaki fragment; lagging strand; replication origin; mcm2-7; orc; leading strand
Journal Title: Molecular Cell
Volume: 65
Issue: 1
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 2017-01-05
Start Page: 131
End Page: 141
Language: English
DOI: 10.1016/j.molcel.2016.10.035
PROVIDER: scopus
PMCID: PMC5256687
PUBMED: 27989437
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008384590&doi=10.1016%2fj.molcel.2016.10.035&partnerID=40&md5=7db12e74afa928df333372f4065be93d
Notes: Article -- Export Date: 2 February 2017 -- Source: Scopus
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MSK Authors
  1. Iestyn Whitehouse
    16 Whitehouse
  2. Ji Qing Jiang
    6 Jiang
  3. Dirk Remus
    21 Remus
  4. Charanya Kumar
    8 Kumar
  5. Sujan Devbhandari
    8 Devbhandari
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