Intrinsic coupling of lagging-strand synthesis to chromatin assembly Journal Article
| Authors: | Smith, D. J.; Whitehouse, I. |
| Article Title: | Intrinsic coupling of lagging-strand synthesis to chromatin assembly |
| Abstract: | Fifty per cent of the genome is discontinuously replicated on the lagging strand as Okazaki fragments. Eukaryotic Okazaki fragments remain poorly characterized and, because nucleosomes are rapidly deposited on nascent DNA, Okazaki fragment processing and nucleosome assembly potentially affect one another. Here we show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the nucleosome repeat. Using deep sequencing, we demonstrate that ligation junctions preferentially occur near nucleosome midpoints rather than in internucleosomal linker regions. Disrupting chromatin assembly or lagging-strand polymerase processivity affects both the size and the distribution of Okazaki fragments, suggesting a role for nascent chromatin, assembled immediately after the passage of the replication fork, in the termination of Okazaki fragment synthesis. Our studies represent the first high-resolution analysis-to our knowledge-of eukaryotic Okazaki fragments in vivo, and reveal the interconnection between lagging-strand synthesis and chromatin assembly. © 2012 Macmillan Publishers Limited. All rights reserved. |
| Keywords: | unclassified drug; sequence analysis; dna-binding proteins; nonhuman; dna replication; polymerase chain reaction; chromosome; protein binding; transcription factors; dna; saccharomyces cerevisiae; eukaryota; genome; saccharomyces cerevisiae proteins; okazaki fragment; dna binding; polydeoxyribonucleotide synthase; synthesis; particle size; ligation; eukaryote; chromatin assembly and disassembly; chromatin structure; dna ligases; nucleosome; nucleosomes; fragmentation; resolution; polydeoxyribonucleotide synthase 1; dna polymerase iii; high-throughput nucleotide sequencing |
| Journal Title: | Nature |
| Volume: | 483 |
| Issue: | 7390 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2012-03-14 |
| Start Page: | 434 |
| End Page: | 438 |
| Language: | English |
| DOI: | 10.1038/nature10895 |
| PROVIDER: | scopus |
| PUBMED: | 22419157 |
| PMCID: | PMC3490407 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 1 May 2012" - "CODEN: NATUA" - "Source: Scopus" |
