Expression of Grb2 distinguishes classical Hodgkin lymphomas from primary mediastinal B-cell lymphomas and other diffuse large B-cell lymphomas Journal Article


Authors: Miles, R. R.; Mankey, C. C.; Seiler, C. E. 3rd; Smith, L. B.; Teruya-Feldstein, J.; Hsi, E. D.; Elenitoba-Johnson, K. S. J.; Lim, M. S.
Article Title: Expression of Grb2 distinguishes classical Hodgkin lymphomas from primary mediastinal B-cell lymphomas and other diffuse large B-cell lymphomas
Abstract: Growth factor receptor-bound protein 2 is an adaptor molecule that mediates B-cell receptor (BCR) signaling pathways, but the expression of growth factor receptor-bound protein 2 in lymphoma tissues has not been reported. We sought to characterize growth factor receptor-bound protein 2 protein expression in reactive tonsillar tissues and lymphoma tissues obtained from diagnostic biopsies of classical Hodgkin lymphoma, primary mediastinal large B-cell lymphoma, diffuse large B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, and 20 low-grade B-cell lymphomas. Growth factor receptor-bound protein 2 expression was assessed in tissues by immunohistochemistry and in lymphoma cell lines by immunoblotting. In reactive lymphoid tissues, growth factor receptor-bound protein 2 was expressed in the cytoplasm of B-cells and histiocytes but not T-cells. Strong, cytoplasmic growth factor receptor-bound protein 2 expression was seen in the neoplastic cells of follicular lymphoma, chronic lymphocytic leukemia/small lymphocytic lymphoma, splenic marginal zone lymphoma, primary mediastinal large B-cell lymphoma, diffuse large B-cell lymphoma, and nodular lymphocyte predominant Hodgkin lymphoma. In contrast, only 10% of the classical Hodgkin lymphomas showed growth factor receptor-bound protein 2 expression in the neoplastic cells. Growth factor receptor-bound protein 2 protein expression was detected by Western blotting in all lymphoma cell lines tested with higher levels in primary mediastinal large B-cell lymphoma compared with classical Hodgkin lymphoma cell lines. These findings support a role for growth factor receptor-bound protein 2 in the diagnostically challenging workup of classical Hodgkin lymphoma versus primary mediastinal large B-cell lymphoma and warrant further studies to evaluate the biologic significance of growth factor receptor-bound protein 2 in the pathogenesis of classical Hodgkin lymphoma. © 2009 Elsevier Inc. All rights reserved.
Keywords: immunohistochemistry; controlled study; human tissue; protein expression; human cell; diagnosis, differential; tumor markers, biological; hodgkin disease; b lymphocyte; b cell lymphoma; blotting, western; lymphoma; immunoblotting; tissue array analysis; histiocyte; large cell lymphoma; lymphoma, large b-cell, diffuse; biopsy technique; mediastinal neoplasms; b-cell; diffuse large b-cell lymphoma; grb2 adaptor protein; hodgkin lymphoma; primary mediastinal large b-cell lymphoma; growth factor receptor bound protein 2; nodular lymphocyte predominant hodgkin lymphoma
Journal Title: Human Pathology
Volume: 40
Issue: 12
ISSN: 0046-8177
Publisher: Elsevier Inc.  
Date Published: 2009-12-01
Start Page: 1731
End Page: 1737
Language: English
DOI: 10.1016/j.humpath.2009.05.009
PUBMED: 19716163
PROVIDER: scopus
DOI/URL:
Notes: --- - "Export Date: 30 November 2010" - "CODEN: HPCQA" - "Source: Scopus"
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  1. Julie T Feldstein
    297 Feldstein