Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation Journal Article

   

Authors:	Ramkumar, N.; Omelchenko, T.; Silva-Gagliardi, N. F.; McGlade, C. J.; Wijnholds, J.; Anderson, K. V.
Article Title:	Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation
Abstract:	During gastrulation of the mouse embryo, individual cells ingress in an apparently stochastic pattern during the epithelial-to-mesenchymal transition (EMT). Here we define a critical role of the apical protein Crumbs2 (CRB2) in the gastrulation EMT. Static and live imaging show that ingressing cells in Crumbs2 mutant embryos become trapped at the primitive streak, where they continue to express the epiblast transcription factor SOX2 and retain thin E-cadherin-containing connections to the epiblast surface that trap them at the streak. CRB2 is distributed in a complex anisotropic pattern on apical cell edges, and the level of CRB2 on a cell edge is inversely correlated with the level of myosin IIB. The data suggest that the distributions of CRB2 and myosin IIB define which cells will ingress, and we propose that cells with high apical CRB2 are basally extruded from the epiblast by neighbouring cells with high levels of apical myosin. © 2016 Macmillan Publishers Limited,part of Springer Nature.All rights reserved.
Journal Title:	Nature Cell Biology
Volume:	18
Issue:	12
ISSN:	1465-7392
Publisher:	Nature Publishing Group
Date Published:	2016-12-01
Start Page:	1281
End Page:	1291
Language:	English
DOI:	10.1038/ncb3442
PROVIDER:	scopus
PUBMED:	27870829
PMCID:	PMC5268168
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996761447&doi=10.1038%2fncb3442&partnerID=40&md5=c6069f70f2be923bca28ed7d84463be5
Notes:	Article -- Export Date: 3 January 2017 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/102934