Radiosynthesis and quality assurance of 5-[(124)I]Iodo-2'-deoxyuridine for functional PET imaging of cell proliferation Journal Article
| Authors: | Guenther, I.; Wyer, L.; Knust, E. J.; Finn, R. D.; Koziorowski, J.; Weinreich, R. |
| Article Title: | Radiosynthesis and quality assurance of 5-[(124)I]Iodo-2'-deoxyuridine for functional PET imaging of cell proliferation |
| Abstract: | 5-[124I]Iodo-2'-deoxyuridine ([124I]IUdR) was routinely produced by direct electrophilic labelling of 2'-deoxyuridine with 124I of high specific activity (12 Ci/μmol) in an Iodogen-coated ReactiVial, followed by purification on a Sep-Pak C-18 cartridge. The radiochemical purity was determined by TLC on a Silicagel-60 plate and by reverse-phase HPLC on a RP- 18 column. Based upon 45 syntheses, the yield ranged from 45% to 65%. The radiochemical impurity of [124I]IUdR was determined at 2.9% by TLC (mainly iodate) and 4.3% by HPLC. The chemical stability of the solvated formulation allowed a time window of 2 days following end of synthesis (EOS) for chemical application, based upon the required 95% radiochemical purity grade of [124I]IUdR. The labelled compound was routinely used for the clinical determination of cell proliferation in glioma patients by positron emission tomography. |
| Keywords: | clinical article; positron emission tomography; glioma; cell proliferation; quality control; cell division; drug synthesis; iodine 125; iodine 131; isotope labeling; iodine radioisotopes; quality assurance; quality assurance, health care; iodine 124; chromatography, high pressure liquid; drug half life; thin layer chromatography; drug stability; drug formulation; tomography, emission-computed; drug purification; iodide; reversed phase high performance liquid chromatography; chromatography, thin layer; idoxuridine; humans; human; article; 5-[124i]iodo-2'-deoxyuridine; radiolabelling; routine production; iodate |
| Journal Title: | Nuclear Medicine and Biology |
| Volume: | 25 |
| Issue: | 4 |
| ISSN: | 0969-8051 |
| Publisher: | Elsevier Science Inc. |
| Date Published: | 1998-05-01 |
| Start Page: | 359 |
| End Page: | 365 |
| Language: | English |
| DOI: | 10.1016/s0969-8051(97)00220-5 |
| PUBMED: | 9639297 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
