Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes Journal Article
| Authors: | Hohl, T. M.; Parlati, F.; Wimmer, C.; Rothman, J. E.; Söllner, T. H.; Engelhardt, H. |
| Article Title: | Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes |
| Abstract: | The structure of 20 S particles, consisting of NSF, SNAPs, and SNARE complexes, was analyzed by electron microscopy and fluorescence resonance energy transfer. Structural changes associated with the binding of α-SNAP and NSF to SNARE complexes define the contribution of each component to the 20 S particle structure. The synaptic SNARE complex forms a 2.5 × 15 nm rod. α-SNAP binds laterally to the rod, increasing its width but not its length. NSF binds to one end of the SNAP/SNARE complex; the resulting 20 S particles measure 22 nm in length and vary in width from 6 nm at their narrowest point to 13.5 nm at their widest. The transmembrane domains of VAMP and syntaxin emerge together at the NSF-distal end of 20 S particles, adjacent to the amino terminus of α-SNAP. |
| Keywords: | carrier protein; genetics; protein conformation; animal; metabolism; animals; nerve tissue proteins; protein binding; membrane proteins; photoprotein; luminescent proteins; immunology; chemistry; hybrid protein; recombinant fusion proteins; brain; myc protein; escherichia coli; carrier proteins; membrane protein; rat; energy transfer; glutathione transferase; dimerization; rats; adenosine triphosphate; proto-oncogene proteins c-myc; isolation and purification; nerve protein; ultrastructure; centrifugation, density gradient; fluorometry; vesicular transport proteins; immunoelectron microscopy; vesicular transport protein; snare protein; syntaxin; qa-snare proteins; density gradient centrifugation; r-snare proteins; snare proteins; microscopy, immunoelectron; synaptobrevin; article; n-ethylmaleimide-sensitive proteins; soluble n-ethylmaleimide-sensitive factor attachment proteins; n ethylmaleimide sensitive factor; soluble n ethylmaleimide sensitive factor attachment protein; nsf protein, rat |
| Journal Title: | Molecular Cell |
| Volume: | 2 |
| Issue: | 5 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 1998-11-01 |
| Start Page: | 539 |
| End Page: | 548 |
| Language: | English |
| PUBMED: | 9844627 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S1097-2765(00)80153-7 |
| PMCID: | PMC5496501 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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