Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes Journal Article
| Authors: | Whiteheart, S. W.; Brunner, M.; Wilson, D. W.; Wiedmann, M.; Rothman, J. E. |
| Article Title: | Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes |
| Abstract: | Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) are required for the binding of N-ethylmaleimide-sensitive fusion protein (NSF) to Golgi membranes and are, therefore, required for intra-Golgi transport. We report the existence of distinct α/β-SNAP and γ-SNAP-binding sites in Golgi membranes that appear to be part of the same receptor complex. Cross- linking studies with α-SNAP demonstrate that an integral membrane protein of between 30-40 kDa is the α-SNAP binding component of the multi-SNAP receptor complex. These data suggest that SNAPs function by independently binding to a multi-SNAP membrane-receptor complex, thereby activating them to serve as adaptors for the targeting of NSF. |
| Keywords: | controlled study; nonhuman; animal; animal tissue; membrane proteins; intracellular transport; liver; amino acid sequence; molecular sequence data; hybrid protein; carrier proteins; membrane protein; rat; binding site; solutions; binding sites; rats; protein cross linking; receptor binding; electrophoresis, polyacrylamide gel; golgi complex; cross-linking reagents; intracellular membranes; membrane receptor; golgi apparatus; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; n ethylmaleimide; ethylmaleimide; liver cell membrane |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 267 |
| Issue: | 17 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1992-06-15 |
| Start Page: | 12239 |
| End Page: | 12243 |
| Language: | English |
| PUBMED: | 1601890 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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