Authors: | Deshpande, R. V.; Moore, M. A. S. |
Article Title: | G-CSF receptor-mediated up-regulation of c-fos but not c-raf mRNA expression in myeloid cells |
Abstract: | Granulocyte colony stimulating factor (G-CSF) regulates survival, proliferation, and differentiation of myeloid precursor cells. One of the signaling mechanisms for the G-CSF receptor (G-CSF-R) involves the activation of Ras/MAP kinase (MEK) pathway. Since Raf is an important, common link between Ras and MEK in the Ras-Raf-MEK cascade, we studied the expression of c-raf mRNA in G-CSF-treated myeloid precursor cell lines - NFS-60 and HL-60. G-CSF did not alter c-raf mRNA expression in these cells up to 24 h, but induced a transient up-regulation of c-fos mRNA expression between 15-60 min post-treatment. Our results show that G-CSF triggers a de novo induction of c-fos but not c-raf mRNA, and suggests that G-CSF-R-mediated activation of Ras/MEK pathway may involve post-transcriptional mechanisms of Raf regulation. |
Keywords: | signal transduction; mitogen activated protein kinase; controlled study; unclassified drug; human cell; polymerase chain reaction; cell proliferation; bone marrow cells; cell survival; cell division; reverse transcription polymerase chain reaction; granulocyte macrophage colony stimulating factor; cell protein; cell differentiation; tumor cells, cultured; stem cell; gene expression regulation; messenger rna; rna, messenger; granulocyte colony stimulating factor receptor; gene control; gene induction; bone marrow cell; receptor; up-regulation; growth factor; granulocyte colony stimulating factor; proto-oncogene proteins c-raf; cytokine receptor; myeloid; protein c fos; proto-oncogene proteins c-fos; hl-60 cells; cell strain hl 60; receptors, granulocyte colony-stimulating factor; humans; human; article; oncogene c fos; protein c raf |
Journal Title: | Molecular and Cellular Biochemistry |
Volume: | 178 |
Issue: | 1-2 |
ISSN: | 0300-8177 |
Publisher: | Springer |
Date Published: | 1998-01-01 |
Start Page: | 47 |
End Page: | 50 |
Language: | English |
DOI: | 10.1023/a:1006894329316 |
PUBMED: | 9546580 |
PROVIDER: | scopus |
DOI/URL: | |
Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |