G-CSF receptor-mediated up-regulation of c-fos but not c-raf mRNA expression in myeloid cells Journal Article

  


Authors: Deshpande, R. V.; Moore, M. A. S.
Article Title: G-CSF receptor-mediated up-regulation of c-fos but not c-raf mRNA expression in myeloid cells
Abstract: Granulocyte colony stimulating factor (G-CSF) regulates survival, proliferation, and differentiation of myeloid precursor cells. One of the signaling mechanisms for the G-CSF receptor (G-CSF-R) involves the activation of Ras/MAP kinase (MEK) pathway. Since Raf is an important, common link between Ras and MEK in the Ras-Raf-MEK cascade, we studied the expression of c-raf mRNA in G-CSF-treated myeloid precursor cell lines - NFS-60 and HL-60. G-CSF did not alter c-raf mRNA expression in these cells up to 24 h, but induced a transient up-regulation of c-fos mRNA expression between 15-60 min post-treatment. Our results show that G-CSF triggers a de novo induction of c-fos but not c-raf mRNA, and suggests that G-CSF-R-mediated activation of Ras/MEK pathway may involve post-transcriptional mechanisms of Raf regulation.
Keywords: signal transduction; mitogen activated protein kinase; controlled study; unclassified drug; human cell; polymerase chain reaction; cell proliferation; bone marrow cells; cell survival; cell division; reverse transcription polymerase chain reaction; granulocyte macrophage colony stimulating factor; cell protein; cell differentiation; tumor cells, cultured; stem cell; gene expression regulation; messenger rna; rna, messenger; granulocyte colony stimulating factor receptor; gene control; gene induction; bone marrow cell; receptor; up-regulation; growth factor; granulocyte colony stimulating factor; proto-oncogene proteins c-raf; cytokine receptor; myeloid; protein c fos; proto-oncogene proteins c-fos; hl-60 cells; cell strain hl 60; receptors, granulocyte colony-stimulating factor; humans; human; article; oncogene c fos; protein c raf
Journal Title: Molecular and Cellular Biochemistry
Volume: 178
Issue: 1-2
ISSN: 0300-8177
Publisher: Springer  
Date Published: 1998-01-01
Start Page: 47
End Page: 50
Language: English
DOI: 10.1023/a:1006894329316
PUBMED: 9546580
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031936335&doi=10.1023%2fA%3a1006894329316&partnerID=40&md5=3f3d8cbd5d44f374b0ca896a1deb080a
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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MSK Authors
  1. Malcolm A S Moore
    549 Moore
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