T cell-dependent immune response in C1q-deficient mice: Defective interferon γ production by antigen-specific T cells Journal Article
| Authors: | Cutler, A. J.; Botto, M.; van Essen, D.; Rivi, R.; Davies, K. A.; Gray, D.; Walport, M. J. |
| Article Title: | T cell-dependent immune response in C1q-deficient mice: Defective interferon γ production by antigen-specific T cells |
| Abstract: | The role of the classical complement pathway in humoral immune responses was investigated in gene-targeted C1q-deficient mice (c1qa(-/-). Production of antigen-specific immunoglobulin (lg)g2a and Igg3 in primary and secondary responses to T cell-dependent antigen was significantly reduced, whereas Igm, Igg1, and Igg2b responses were similar in control and C1qa(-/-) mice. Despite abnormal humoral responses, B cells from C1qa(-/-) mice proliferated normally to a number of stimuli in vitro. Immune complex localization to follicular dendritic cells within splenic follicles was lacking in C1qa(-/-) mice. The precursor frequency of antigen-specific T cells was similar in C1qa(-/-) and wild-type mice. However, analysis of cytokine production by primed T cells in response to keyhole limpet hemocyanin revealed a significant reduction in interferon-γ production in C1qa(-/-) mice compared with control mice, whereas interleukin 4 secretion was equivalent. These data suggest that the classical pathway of complement may influence the cytokine profile of antigen-specific T lymphocytes and the subsequent immune response. |
| Keywords: | nonhuman; t-lymphocytes; animal cell; mouse; animals; mice; gene targeting; cell division; mice, inbred c57bl; b-lymphocytes; antigen presentation; immune response; antigens; gamma interferon; antigen specificity; antibody response; antibodies; memory cell; t lymphocyte activation; antibody production; deficiency; interleukin-4; antigen antibody complex; interferon type ii; b lymphocyte activation; antigen-antibody complex; male; female; priority journal; article; complement component c1q; complemen; interferon γ; complement deficiency; complement c1q; complement pathway, classical |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 187 |
| Issue: | 11 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1998-06-01 |
| Start Page: | 1789 |
| End Page: | 1797 |
| Language: | English |
| DOI: | 10.1084/jem.187.11.1789 |
| PUBMED: | 9607920 |
| PROVIDER: | scopus |
| PMCID: | PMC2212306 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
