Dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can initiate antigen-specific humoral immunity independent of CD4+ T cells Journal Article
| Authors: | Kikuchi, T.; Worgall, S.; Singh, R.; Moore, M. A. S.; Crystal, R. G. |
| Article Title: | Dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can initiate antigen-specific humoral immunity independent of CD4+ T cells |
| Abstract: | We have investigated whether dendritic cells genetically modified to express CD40 ligand and pulsed with antigen can trigger B cells to produce antigen-specific antibodies without CD4+ T-cell help. Dendritic cells modified with a recombinant adenovirus vector to express CD40 ligand and pulsed with heat-killed Pseudomonas induced naive B cells to produce antibodies against Pseudomonas in the absence of CD4+ T cells in vitro, initiated Pseudomonas-specific humoral immune responses in vivo in wild-type and CD4(-/-) mice, and protected immunized wild-type and CD4(-/-), but not B-cell(-/-) mice, from lethal intrapulmonary challenge with Pseudomonas. Thus, genetic modification of dendritic cells with CD40 ligand enables them to present a complex mixture of microbial antigens and establish CD4+ T cell-independent, B cell-mediated protective immunity against a specific microbe. |
| Keywords: | human cell; binding affinity; animals; mice; dendritic cell; cd40 ligand; immunoglobulin; mice, inbred c57bl; b-lymphocytes; genetic vectors; monoclonal antibody; cytokine; cytokines; dendritic cells; immune response; genetic engineering; antigen specificity; cd4-positive t-lymphocytes; antibody specificity; humoral immunity; cell interaction; ligand binding; helper cell; virus vector; immunization; coculture techniques; pseudomonas aeruginosa; pseudomonas; bactericidal activity; human; female; priority journal; article; pseudomonas infections |
| Journal Title: | Nature Medicine |
| Volume: | 6 |
| Issue: | 10 |
| ISSN: | 1078-8956 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2000-10-01 |
| Start Page: | 1154 |
| End Page: | 1159 |
| Language: | English |
| DOI: | 10.1038/80498 |
| PUBMED: | 11017148 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
