Identification and differential regional expression of KOR-3/ORL-1 gene splice variants in mouse brain Journal Article
| Authors: | Pan, Y. X.; Xu, J.; Wan, B. L.; Zuckerman, A.; Pasternak, G. W. |
| Article Title: | Identification and differential regional expression of KOR-3/ORL-1 gene splice variants in mouse brain |
| Abstract: | KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deletion at the 3'-end of the first coding exon (KOR-3d) and an 81 bp insertion between the second and third coding exons (KOR-3e), three new variants with insertions of 34 (KOR-3a), 98 (KOR-3b), and 139 bp (KOR-3c) between the first and second coding exons have been obtained. The expression of the three variants in mouse brain varies markedly among brain regions with a distribution which is quite distinct from KOR-3 itself. Of greatest interest was the presence of high levels of KOR-3a in the striatum, a region with no demonstrable KOR-3, and in the cortex. KOR-3c was seen in the periaqueductal gray and hypothalamus, regions where KOR-3 predominated. The brainstem had similar levels of KOR-3, KOR-3a, and KOR-3d. In contrast, KOR-3d was most prominent in the cerebellum. KOR-3b levels were very low throughout. Copyright (C) 1998 Federation of European Biochemical Societies. |
| Keywords: | exon; gene deletion; nonhuman; mouse; animals; mice; animal tissue; gene expression; nerve tissue proteins; mice, inbred c57bl; gene expression regulation; cloning, molecular; molecular sequence data; brain; organ specificity; alternative splicing; alternative rna splicing; murinae; base sequence; blotting, northern; opiate receptor; dna, complementary; opioid receptor; splicing; brain tissue; kappa opiate receptor; nociceptin; brain chemistry; kappa3 receptor; receptors, opioid; priority journal; article; receptors, opioid, kappa; staphylococcus phage 3a; orphanin fq; kor-3; orl-1 |
| Journal Title: | FEBS Letters |
| Volume: | 435 |
| Issue: | 1 |
| ISSN: | 0014-5793 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1998-09-11 |
| Start Page: | 65 |
| End Page: | 68 |
| Language: | English |
| DOI: | 10.1016/s0014-5793(98)01039-4 |
| PUBMED: | 9755860 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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