Apoptosis in mouse embryos: Elevated levels in pregastrulae and in the distal anterior region of gastrulae of normal and mutant mice Journal Article

  

Authors:	Manova, K.; Tomihara-Newberger, C.; Wang, S.; Godelman, A.; Kalantry, S.; Witty-Blease, K.; De Leon, V.; Chen, W. S.; Lacy, E.; Bachvarova, R. F.
Article Title:	Apoptosis in mouse embryos: Elevated levels in pregastrulae and in the distal anterior region of gastrulae of normal and mutant mice
Abstract:	The pattern of apoptotic cell death has been surveyed in prestreak and primitive streak embryos of four strains of mice and in three mutants affecting gastrulation. In C57BL/6 embryos, a high level of cell death occurs in the early egg cylinder stage at embryonic day 5 (E5) to E5.5. In all strains, cell death is elevated shortly before gastrulation, but the level varies four- to fivefold among strains. During gastrulation, cell death declines but is relatively more abundant in the distal and distal anterior regions. Early streak embryos cultured in media with reduced levels of growth factors show increased cell death mainly in the distal region. In three mutants with disturbed function of the proximal visceral endoderm and/or primitive streak, cell death is increased, and the regional pattern seen in normal embryos is intensified. The results strongly suggest that the proximal visceral endoderm and primitive streak region are the principal sites of synthesis of growth factors promoting cell survival. We conclude that localized growth factor supply has an important role in regulating the size of the embryo and of embryonic regions.
Keywords:	gene mutation; mutation; dna-binding proteins; nonhuman; animal cell; mouse; animals; mice; cell death; cell survival; cell division; apoptosis; embryo; mice, mutant strains; mice, inbred icr; mice, inbred c57bl; time factors; transcription factors; gene expression regulation, developmental; phosphoproteins; pregnancy; growth factor; endoderm; gastrulation; mutants; growth substances; neuroectoderm; primitive streak; in situ nick-end labeling; gastrula; male; female; priority journal; article; embryonic and fetal development; hepatocyte nuclear factor 4
Journal Title:	Developmental Dynamics
Volume:	213
Issue:	3
ISSN:	1058-8388
Publisher:	Wiley Periodicals, Inc
Date Published:	1998-11-01
Start Page:	293
End Page:	308
Language:	English
DOI:	10.1002/(sici)1097-0177(199811)213:3<293::aid-aja6>3.0.co;2-d
PUBMED:	9825865
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-7844229411&doi=10.1002%2f%28SICI%291097-0177%28199811%29213%3a3%3c293%3a%3aAID-AJA6%3e3.0.CO%3b2-D&partnerID=40&md5=876221c4b5b6069a1064f81aab432cf1
Notes:	Article -- Export Date: 12 December 2016 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/102299