Expression of potential target antigens for immunotherapy on primary and metastatic prostate cancers Journal Article
| Authors: | Zhang, S.; Zhang, H. S.; Reuter, V. E.; Slovin, S. F.; Scher, H. I.; Livingston, P. O. |
| Article Title: | Expression of potential target antigens for immunotherapy on primary and metastatic prostate cancers |
| Abstract: | Defining the expression of tumor-associated antigens on primary and metastatic prostate cancer is the crucial first step in selecting appropriate targets for immune attack. In this study, the distribution of the tumor- associated antigens GM2, Tn, sTn, Thompson-Friedenreich antigen (TF), Globo H, Le(y), MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC7, carcinoembryonic antigen, β chain of human chorionic gonadotropin (hCGβ), HER2/neu, PSMA, and KSA on primary and metastatic prostate cancer and 16 types of normal tissues was compared by immunohistochemistry, using a panel of well- characterized monoclonal antibodies. Our results show that GM2, KSA, and MUC2 were strongly expressed on 8 or 9 of 9 metastatic prostate cancer biopsy specimens and, with PSMA, hCGβ, TF, Tn, and sTn, on 8 or more of 11 primary prostate cancer specimens. Tn, MUC1, and PSMA were expressed on 4-6 of 9 metastatic specimens. The remaining antigens were expressed on no more than three of nine metastatic specimens. Normal tissues were also tested with all antibodies. With regard to the eight antigens most widely expressed on prostate cancers, PSMA was not expressed significantly on any of the normal tissues except prostate epithelium. Tn, sTn, hCGβ, and MUC2 were detected on up to 3 of 10 types of normal epithelia. GM2, TF, MUC1, and KSA were more broadly distributed on normal epithelia, all primarily at the secretory borders. STn, KSA, and hCGβ were also detected in the testis, and GM2 was expressed on gray matter of brain. From the 30 antigens that we have screened, this study provides the basis for selecting GM2, TF, Tn, sTn, hCGβ, MUC1, MUC2, KSA, and PSMA as target antigens for specific immunotherapy of prostate cancer. |
| Keywords: | immunohistochemistry; controlled study; human tissue; oncoprotein; antigen expression; metastasis; carcinoembryonic antigen; tumor biopsy; tumor antigen; prostate cancer; prostatic neoplasms; antibodies, monoclonal; amino acid sequence; molecular sequence data; immunotherapy; antigens, neoplasm; tissue distribution; cancer vaccines; antigen detection; prostate biopsy; neoplasm metastasis; ganglioside gm2; carbohydrate sequence; chorionic gonadotropin beta subunit; antigens, surface; epitopes; humans; human; male; priority journal; article |
| Journal Title: | Clinical Cancer Research |
| Volume: | 4 |
| Issue: | 2 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1998-02-01 |
| Start Page: | 295 |
| End Page: | 302 |
| Language: | English |
| PUBMED: | 9516914 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
Citation Impact
MSK Authors
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254Slovin -
1228Reuter -
228Livingston -
1130Scher -
25Zhang
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