SNAREpins: Minimal machinery for membrane fusion Journal Article
| Authors: | Weber, T.; Zemelman, B. V.; McNew, J. A.; Westermann, B.; Gmachl, M.; Parlati, F.; Söllner, T. H.; Rothman, J. E. |
| Article Title: | SNAREpins: Minimal machinery for membrane fusion |
| Abstract: | Recombinant v- and t-SNARE proteins reconstituted into separate lipid bilayer vesicles assemble into SNARE-pins-SNARE complexes linking two membranes. This leads to spontaneous fusion of the docked membranes at physiological temperature. Docked unfused intermediates can accumulate at lower temperatures and can fuse when brought to physiological temperature. A supply of unassembled v- and t-SNAREs is needed for these intermediates to form, but not for the fusion that follows. These data imply that SNARE-pins are the minimal machinery for cellular membrane fusion. |
| Keywords: | complex formation; nerve tissue proteins; membrane proteins; bacterial proteins; escherichia coli; recombinant proteins; cell membrane; dna, bacterial; lipid bilayers; lipid bilayer; lipid vesicle; cell fusion; vesicular transport proteins; intracellular membranes; snare proteins; priority journal; article |
| Journal Title: | Cell |
| Volume: | 92 |
| Issue: | 6 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1998-03-20 |
| Start Page: | 759 |
| End Page: | 772 |
| Language: | English |
| DOI: | 10.1016/s0092-8674(00)81404-x |
| PUBMED: | 9529252 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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