Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation Journal Article
| Authors: | McGuirk, J.; Hao, G.; Hou, W.; Abhyankar, S.; Williams, C.; Yan, W.; Yuan, J.; Guan, X.; Belt, R.; Dejarnette, S.; Wieman, J.; Yan, Y. |
| Article Title: | Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantation |
| Abstract: | We studied serum proteomic profiling in patients with graft versus host disease (GVHD) after allogeneic hematopoietic cell transplantation (allo-HCT) by two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. The expression of a group of proteins, haptoglobin (Hp), alpha-1-antitrypsin, apolipoprotein A-IV, serum paraoxonase and Zn-alpha-glycoprotein were increased and the proteins, clusterin precursor, alpha-2-macroglobulin, serum amyloid protein precursor, sex hormone-binding globulin, serotransferrin and complement C4 were decreased in patients with extensive chronic GVHD (cGVHD). Serum haptoglobin (Hp) levels in patients with cGVHD were demonstrated to be statistically higher than in patients without cGVHD and normal controls (p<0.01). We used immunoblotting and PCR in combination with 2-DE gel image analysis to determine Hp polymorphisms in 25 allo-HCT patients and 16 normal donors. The results demonstrate that patients with cGVHD had a higher incidence of HP 2-2 phenotype (43.8%), in comparison to the patients without cGVHD (0%) and normal donors (18.7%), suggesting the possibility that specific Hp polymorphism may play a role in the development of cGVHD after allo-HCT. In this study, quantitative serum Hp levels were shown to be related to cGVHD development. Further, the data suggest the possibility that specific Hp polymorphisms may be associated with cGVHD development and warrant further investigation. © 2009 McGuirk et al; licensee BioMed Central Ltd. |
| Keywords: | adult; clinical article; controlled study; protein expression; middle aged; transplantation, homologous; young adult; unclassified drug; acute granulocytic leukemia; single nucleotide polymorphism; case control study; genetics; case-control studies; busulfan; prednisone; disease course; myeloid metaplasia; cytarabine; methotrexate; molecular genetics; polymerase chain reaction; mass spectrometry; protein blood level; blood proteins; phenotype; metabolism; multiple myeloma; gene expression profiling; incidence; genotype; hematopoietic stem cell transplantation; chronic myeloid leukemia; proteomics; acute lymphoblastic leukemia; physiology; blood; acute graft versus host disease; chronic graft versus host disease; myelodysplastic syndrome; nonhodgkin lymphoma; whole body radiation; amino acid sequence; molecular sequence data; heart failure; nucleotide sequence; graft versus host reaction; immunoblotting; allogeneic hematopoietic stem cell transplantation; cancer relapse; tandem mass spectrometry; chromatography, liquid; tacrolimus; graft vs host disease; chronic lymphatic leukemia; liquid chromatography; sex hormone binding globulin; polymorphism, genetic; haptoglobin; glycoprotein; allotransplantation; thymocyte antibody; aplastic anemia; alpha 1 antitrypsin; alpha 2 macroglobulin; amyloid precursor protein; apolipoprotein a4; aryldialkylphosphatase; clusterin; complement component c4; serotransferrin; transferrin; zinc alpha glycoprotein; hp protein, human; plasma protein; two dimensional gel electrophoresis; genetic polymorphism; haptoglobins |
| Journal Title: | Journal of Hematology & Oncology |
| Volume: | 2 |
| ISSN: | 1756-8722 |
| Publisher: | Biomed Central Ltd |
| Date Published: | 2009-04-20 |
| Start Page: | 17 |
| Language: | English |
| DOI: | 10.1186/1756-8722-2-17 |
| PUBMED: | 19379511 |
| PROVIDER: | scopus |
| PMCID: | PMC2678154 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "Art. No.: 17" - "Source: Scopus" |
