TNFα disrupts mitotic clonal expansion and regulation of retinoblastoma proteins p130 and p107 during 3T3-L1 adipocyte differentiation Journal Article


Authors: Lyle, R. E.; Richon, V. M.; McGehee, R. E. Jr
Article Title: TNFα disrupts mitotic clonal expansion and regulation of retinoblastoma proteins p130 and p107 during 3T3-L1 adipocyte differentiation
Abstract: The inhibitory effects of TNFα on adipocyte differentiation are well described, however, the mechanisms are poorly understood. Early during hormonally-induced 3T3-L1 preadipocyte differentiation there is a requisite mitotic clonal expansion phase that is associated with significant regulation in p130 and p107 protein levels, two members of the retinoblastoma protein family that regulate cell cycle events through interactions with the E2F transcription factors. This regulation occurs within the first 24 hours of differentiation (Day 1) and is characterized by a transient increase in p107 protein and mRNA levels as well as a transient decrease in p130 protein levels. Here we describe that TNFα disrupts the normal pattern of expression of both p130 and p107 proteins, leading to a complete block in mitotic clonal expansion. Interestingly TNFα-treated cells enter S-phase as determined by 5-bromo-2@?-deoxyuridine uptake experiments, but rather than completing cell cycle, they are stimulated to undergo apoptosis.
Keywords: immunohistochemistry; controlled study; genetics; nonhuman; mitosis; proteins; animal cell; mouse; animal; cytology; metabolism; animals; mice; cell cycle s phase; apoptosis; nuclear protein; protein; cell differentiation; drug effect; nuclear proteins; chemistry; tumor necrosis factor alpha; tumor necrosis factor-alpha; messenger rna; rna, messenger; phosphoproteins; cell clone; retinoblastoma protein; molecular weight; protein p107; protein p130; retinoblastoma-like protein p107; retinoblastoma-like protein p130; phosphoprotein; cell strain 3t3; 3t3 cells; cell labeling; broxuridine; adipocyte; adipocytes; proadipocyte; priority journal; article; rbl1 protein, mouse; rbl2 protein, mouse
Journal Title: Biochemical and Biophysical Research Communications
Volume: 247
Issue: 2
ISSN: 0006-291X
Publisher: Elsevier Science, Inc.  
Date Published: 1998-06-18
Start Page: 373
End Page: 378
Language: English
DOI: 10.1006/bbrc.1998.8773
PUBMED: 9642134
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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  1. Victoria M Richon
    88 Richon