Functional analysis of pRb2/p130 interaction with cyclins Journal Article
| Authors: | Claudio, P. P.; De Luca, A.; Howard, C. M.; Baldi, A.; Firpo, E. J.; Koff, A.; Paggi, M. G.; Giordano, A. |
| Article Title: | Functional analysis of pRb2/p130 interaction with cyclins |
| Abstract: | The retinoblastoma (Rb) family consists of the tumor suppressor pRb and related proteins p107 and pRb2/p130. Ectopic expression of pRb and p107 results in a growth arrest of sensitive cells in the G1 phase of the cell cycle. We demonstrated here that the growth-suppressive properties of pRb2/p130 were also specific for the G1 phase. The A-, E-, and D-type cyclins as well as transcription factor E2F1 and the E1A viral oncoprotein were able to rescue the pRb2/p130-mediated G1 growth arrest in SAOS-2 cells. The rescue with cyclins A and E correlated with their physical interaction with pRb2/p130, which surprisingly has been found to occur over all phases of the cell cycle. The phosphorylation status as well as the kinase activity associated with pRb2/p130 dramatically increased near the G1-S-phase transition. This suggests that, like the other Rb family members, pRb and p107, the phosphorylation of pRb2/p130 is controlled by the cell cycle machinery and that pRb2/p130 may indeed be another key G1-S-phase regulator. |
| Keywords: | protein phosphorylation; human cell; flow cytometry; proteins; cell cycle; cell cycle s phase; retinoblastoma; tumor cells, cultured; transfection; phosphorylation; gene expression regulation; tumor suppressor gene; protein-serine-threonine kinases; growth regulation; phosphoproteins; cyclin-dependent kinases; cyclins; retinoblastoma protein; cell cycle g1 phase; g1 phase; retinoblastoma-like protein p130; s phase; protein dna interaction; growth inhibition; cyclin-dependent kinase 2; tumor stem cell assay; cdc2-cdc28 kinases; humans; human; priority journal; article |
| Journal Title: | Cancer Research |
| Volume: | 56 |
| Issue: | 9 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1996-05-01 |
| Start Page: | 2003 |
| End Page: | 2008 |
| Language: | English |
| PUBMED: | 8616840 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
