Generating late-onset human iPSC-based disease models by inducing neuronal age-related phenotypes through telomerase manipulation Journal Article
| Authors: | Vera, E.; Bosco, N.; Studer, L. |
| Article Title: | Generating late-onset human iPSC-based disease models by inducing neuronal age-related phenotypes through telomerase manipulation |
| Abstract: | Modeling late-onset disorders such as Parkinson's disease (PD) using iPSC technology remains a challenge, as current differentiation protocols yield cells with the properties of fetal-stage cells. Here, we tested whether it is possible to accelerate aging in vitro to trigger late-onset disease phenotypes in an iPSC model of PD. In order to manipulate a factor that is involved in natural aging as well as in premature aging syndromes, we used telomere shortening as an age-inducing tool. We show that shortened telomeres result in age-associated as well as potentially disease-associated phenotypes in human pluripotent stem cell (hPSC)-derived midbrain dopamine (mDA) neurons. Our approach provides proof of concept for the further validation of telomere shortening as an induced-aging tool for late-onset-disease modeling. © 2016 The Authors |
| Keywords: | telomerase; aging; disease modeling; ipsc; telomeres; induced aging; late-onset disease; neuronal aging |
| Journal Title: | Cell Reports |
| Volume: | 17 |
| Issue: | 4 |
| ISSN: | 2211-1247 |
| Publisher: | Cell Press |
| Date Published: | 2016-10-18 |
| Start Page: | 1184 |
| End Page: | 1192 |
| Language: | English |
| DOI: | 10.1016/j.celrep.2016.09.062 |
| PROVIDER: | scopus |
| PMCID: | PMC5089807 |
| PUBMED: | 27760320 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 December 2016 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work