Chromosomal and proteome analysis of a new T24-based cell line model for aggressive bladder cancer Journal Article
| Authors: | Makridakis, M.; Gagos, S.; Petrolekas, A.; Roubelakis, M. G.; Bitsika, V.; Stravodimos, K.; Pavlakis, K.; Anagnou, N. P.; Coleman, J.; Vlahou, A. |
| Article Title: | Chromosomal and proteome analysis of a new T24-based cell line model for aggressive bladder cancer |
| Abstract: | Cell line models aid in understanding cancer aggressiveness. The aim of this study was the establishment of a metastatic variant (T24M) of the T24 bladder cancer cell line and its initial characterization at chromosomal and proteomic levels. T24M were spontaneously developed in mice from T24 cells, following cycles of subcutaneous injections and culture in vitro. Transwell migration assays and injections in mice revealed increased migration and tumorigenic properties of T24M compared to the T24 cells. Cytogenetic analysis demonstrated that T24M retained several karyotypic characteristics of the parental cells and also acquired novel chromosomal aberrations related to aggressive bladder cancer. Proteomic analysis of the T24 and T24M cells by 2-DE and MS led to the generation of their 2-DE proteomic map and revealed differences in multiple proteins. These include proteases of the lysosomal and proteasome degradation pathways, mitochondrial and cytoskeletal proteins. The 2-DE findings were confirmed by immunoblotting of cell lysates and immunohistochemistry of bladder cancer tissue sections for cathepsin D and activity assays for proteasome. Collectively, our results suggest that the T24M cells reflect many known chromosomal and proteomic aberrations encountered in aggressive bladder cancers but also provide access to novel findings with potentially clinical applications. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA. |
| Keywords: | immunohistochemistry; controlled study; human tissue; protein expression; human cell; cell proliferation; proteins; proteome; animals; mice; mus; proteasome; proteasome endopeptidase complex; protein degradation; cytogenetics; cancer cell culture; in vitro study; mice, scid; enzyme activity; cell line, tumor; proteomics; bladder cancer; urinary bladder neoplasms; chromosome aberration; gene expression regulation, neoplastic; nucleotide sequence; urinary bladder; immunoblotting; cell migration; cell movement; chi-square distribution; proteinase; cancer tissue; neoplasm transplantation; chromosome aberrations; disease models, animal; electrophoresis, gel, two-dimensional; chromosome analysis; karyotype; cytoskeleton; statistics, nonparametric; mitochondrion; bladder carcinogenesis; cytogenetic analysis; 2-de; metastatic variant; t24 cell line; cathepsin d; cytoskeleton protein; cell lysate; lysosome; chromosome painting |
| Journal Title: | Proteomics |
| Volume: | 9 |
| Issue: | 2 |
| ISSN: | 1615-9853 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2009-01-01 |
| Start Page: | 287 |
| End Page: | 298 |
| Language: | English |
| DOI: | 10.1002/pmic.200800121 |
| PUBMED: | 19105184 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 30 November 2010" - "CODEN: PROTC" - "Source: Scopus" |
