Interlocked mismatch-aligned arrowhead DNA motifs Journal Article
| Authors: | Kettani, A.; Bouaziz, S.; Skripkin, E.; Majumdar, A.; Wang, W. M.; Jones, R. A.; Patel, D. J. |
| Article Title: | Interlocked mismatch-aligned arrowhead DNA motifs |
| Abstract: | Background: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion, The all-purine d(GGA)(n) triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation, Results: We have solved the structure of the uniformly C-13,N-15-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation, The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. Conclusions: The purine-rich (GGA)(n) triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)(2) triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases. |
| Keywords: | human-disease; crystal-structure; nmr; sequences; repeats; base-pairs; dynamic mutations; uniform c-13,n-15-labeled dna; (gga) triplet repeat; mismatch alignments; parallel-stranded segments; v-shaped backbone; triple-helix formation; parallel-stranded dna; dot-a pairs |
| Journal Title: | Structure |
| Volume: | 7 |
| Issue: | 7 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 1999-07-15 |
| Start Page: | 803 |
| End Page: | 815 |
| Language: | English |
| ACCESSION: | WOS:000081492400010 |
| DOI: | 10.1016/s0969-2126(99)80104-x |
| PROVIDER: | wos |
| PUBMED: | 10425682 |
| Notes: | Article -- Source: Wos |
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