Characterization of two novel sublines established from a human megakaryoblastic leukemia cell line transfected with p210(BCR-ABL) Journal Article
| Authors: | Berman, E.; Jhanwar, S.; McBride, M.; Strife, A.; Wisniewski, D.; Lambek, C.; Clarkson, B. |
| Article Title: | Characterization of two novel sublines established from a human megakaryoblastic leukemia cell line transfected with p210(BCR-ABL) |
| Abstract: | Disease progression in chronic myelogenous leukemia (CML) is usually accompanied by chromosomal abnormalities such as an additional Ph chromosome, trisomies of chromosome 8 or 19, or i(17) in addition to the standard translocation t(9;22) (q34;q11). However, detailed studies of the various steps involved during this evolution are difficult to perform, thereby making the study of cell lines that contain the transposed genes BCR-ABL, especially those of human origin, an important focus. In this analysis we investigated the human megakaryoblastic cell line MO7e and its subline transfected with BCR-ABL, MO7e/p210. Initial studies demonstrated that the phenotype of the MO7e line was consistent with a megakaryocytic lineage as originally described and was growth factor dependent in liquid culture. The MO7e/p210 subline, however, was growth factor independent and could be further separated into two distinct sublines based on expression of glycophorin A using the monoclonal antibody R10. The subline R10 negative (R10-) was similar to the parent line MO7e but R10 positive (R10+) cells had a distinct erythroid phenotype. In addition, the R10- and R10+ sublines demonstrated strikingly different colony morphology when cultured in semisolid medium. Furthermore, R10+ cells had additional chromosomal abnormalities not detected in the R10- population. These results demonstrate that the insertion of the BCR-ABL in this human leukemia cell line resulted in two distinct subpopulations of cells, each now growth factor independent, but one with a phenotype and karyotype identical to the parent cell line and the other with a different phenotype and additional chromosomal abnormalities. These two subpopulations derived from the MO7e/p210 transfected cell line may prove useful in further understanding the multistep events that occur in the progression of this disease. Copyright (C) 2000 Elsevier Science Ltd. |
| Keywords: | controlled study; human cell; disease course; phenotype; cell line; cytogenetics; tumor cells, cultured; transfection; chronic myeloid leukemia; chromosome aberration; genetic transfection; cell culture; leukemia cell; cell subpopulation; immunophenotyping; chromosome aberrations; fusion proteins, bcr-abl; restriction fragment length polymorphism; polymorphism, restriction fragment length; leukemia, megakaryocytic, acute; humans; human; priority journal; article; glycophorin a; cml evolution; ph+ transfected cell line |
| Journal Title: | Leukemia Research |
| Volume: | 24 |
| Issue: | 4 |
| ISSN: | 0145-2126 |
| Publisher: | Elsevier Ltd |
| Date Published: | 2000-04-01 |
| Start Page: | 289 |
| End Page: | 297 |
| Language: | English |
| DOI: | 10.1016/s0145-2126(99)00179-4 |
| PUBMED: | 10713326 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 23 September 2016 -- Source: Scopus |
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