The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum ΔH contains DNA helicase activity Journal Article

   


Authors: Kelman, Z.; Lee, J. K.; Hurwitz, J.
Article Title: The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum ΔH contains DNA helicase activity
Abstract: Previous studies have identified an ATP-dependent DNA helicase activity intrinsic to the human minichromosome maintenance (MCM) complex, composed of MCM subunits 4, 6, and 7 [Ishimi, Y. (1997) J. Biol. Chem. 272, 24508-24513]. In contrast to the presence of multiple MCM genes (at least six) in eukaryotes, the archaeon Methanobacterium thermoautotrophicum ΔH (mth) genome contains a single open reading frame coding for an MCM protein. In this study we report the isolation of the mthMCM protein overexpressed in Escherichia coli. The purified recombinant protein was found to exist in both multimeric (≃103 kDa) and monomeric (76 kDa) forms. Both forms of the protein bind to single-stranded DNA, hydrolyze ATP in the presence of DNA, and possess 3'-to-5' ATP-dependent DNA helicase activities. Thus, a single mthMCM protein contains biochemical properties identical to those associated with the eukaryotic MCM4, -6, and -7 complex. These results suggest that the characterization of the mthMCM protein and its multiple forms may contribute to our understanding of the role of MCM helicase activity in eukaryotic chromosomal DNA replication.
Keywords: dna-binding proteins; nonhuman; dna replication; protein conformation; cell cycle proteins; gene overexpression; protein dna binding; enzyme activity; bacteria (microorganisms); nuclear proteins; bacterial protein; eukaryota; escherichia coli; recombinant protein; helicase; saccharomyces cerevisiae proteins; adenosine triphosphate; single stranded dna; dna, single-stranded; adenosine triphosphatases; dna helicases; archaea; archaeal proteins; protein isolation; replication; open reading frame; prokaryota; eukaryotic cells; actinobacteria (class); minichromosome; methanobacterium thermoautotrophicum; dna, archaeal; methanobacterium; priority journal; article; uncultured actinomycete; methanothermobacter thermautotrophicus; cdc6 protein; orc1 protein; chromosomes, archaeal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 96
Issue: 26
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1999-12-21
Start Page: 14783
End Page: 14788
Language: English
DOI: 10.1073/pnas.96.26.14783
PUBMED: 10611290
PROVIDER: scopus
PMCID: PMC24725
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033593053&partnerID=40&md5=98d4f6e5f6727758b1ed0fa6200d38e5
Notes: Article -- Export Date: 16 August 2016 -- Source: Scopus
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MSK Authors
  1. Joon-Kyu Lee
    9 Lee
  2. Jerard Hurwitz
    206 Hurwitz
  3. Zvi Kelman
    17 Kelman
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