High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T Journal Article
| Authors: | Sundin, J.; Tolmachev, V.; Koziorowski, J.; Carlsson, J.; Lundqvist, H.; Welt, S.; Larson, S.; Sundin, A. |
| Article Title: | High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T |
| Abstract: | Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography. |
| Keywords: | immunohistochemistry; controlled study; human cell; positron emission tomography; cancer diagnosis; radiopharmaceuticals; diagnostic approach route; tumor cells, cultured; immunoreactivity; monoclonal antibodies; monoclonal antibody; antibodies, monoclonal; isotope labeling; cancer cell; pet; tosyl compounds; hydrogen-ion concentration; indicators and reagents; bromination; bromine radioisotopes; a33; buffers; bromine derivative; tosylchloramide sodium; human; article; 76br; cat-labeling; radiobromination; chloramines |
| Journal Title: | Nuclear Medicine and Biology |
| Volume: | 26 |
| Issue: | 8 |
| ISSN: | 0969-8051 |
| Publisher: | Elsevier Science Inc. |
| Date Published: | 1999-11-01 |
| Start Page: | 923 |
| End Page: | 929 |
| Language: | English |
| DOI: | 10.1016/s0969-8051(99)00076-1 |
| PUBMED: | 10708306 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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