A general approach to the non-invasive imaging of transgenes using Cis-linked herpes simplex virus thymidine kinase Journal Article
| Authors: | Tjuvajev, J. G.; Joshi, A.; Callegari, J.; Lindsley, L.; Joshi, R.; Balatoni, J.; Finn, R.; Larson, S. M.; Sadelain, M.; Blasberg, R. G. |
| Article Title: | A general approach to the non-invasive imaging of transgenes using Cis-linked herpes simplex virus thymidine kinase |
| Abstract: | Non-invasive imaging of gene expression opens new prospects for the study of transgenic animals and the implementation of genetically based therapies in patients. We have sought to establish a general paradigm to enable whole body non-invasive imaging of any transgene. We show that the expression and imaging of HSV1-tk (a marker gene) can be used to monitor the expression of the LacZ gene (a second gene) under the transcriptional control of a single promoter within a bicistronic unit that includes a type II internal ribosomal entry site. In cells bearing a single copy of the vector, the expression of the two genes is proportional and constant, both in vitro and in vivo. We demonstrate that non-invasive imaging of HSV1-tk gene accurately reflects the topology and activity of the other cis-linked transgene. |
| Keywords: | promoter region; genetics; methodology; animal; metabolism; animals; genetic transcription; transcription, genetic; enzymology; tumor cells, cultured; diagnostic imaging; gene vector; genetic transduction; genetic vectors; transduction, genetic; cell culture; escherichia coli; rat; gene therapy; transgene; beta galactosidase; simplexvirus; thymidine kinase; rats; neoplasm transplantation; rats, sprague-dawley; tumor; herpes simplex virus; sprague dawley rat; transgenes; retrovirus; retroviridae; hsv1-tk; cancer transplantation; lactose operon; lac operon; beta-galactosidase; southern blotting; gamma radiation; gamma rays; promoter regions (genetics); blotting, southern; gene imaging; article; ires |
| Journal Title: | NeoPlasia |
| Volume: | 1 |
| Issue: | 4 |
| ISSN: | 1522-8002 |
| Publisher: | Elsevier Science Inc. |
| Date Published: | 1999-10-01 |
| Start Page: | 315 |
| End Page: | 320 |
| Language: | English |
| PUBMED: | 10935486 |
| PROVIDER: | scopus |
| PMCID: | PMC1508100 |
| DOI: | 10.1038/sj.neo.7900053 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
