FAST-2 is a mammalian winged-helix protein which mediates transforming growth factor β signals Journal Article
| Authors: | Liu, B.; Dou, C. L.; Prabhu, L.; Lai, E. |
| Article Title: | FAST-2 is a mammalian winged-helix protein which mediates transforming growth factor β signals |
| Abstract: | The mechanisms by which transforming growth factor β (TGF-β) and related ligands regulate transcription remain poorly understood. The winged- helix (WH) transcription factor fork head activin signal transducer 1 (FAST- 1) was identified as a mediator of activin signaling in Xenopus embryos (X. Chen, M. J. Rubock, and M. Whitman, Nature 383:691-696, 1996). We have cloned a novel WH gene from the mouse which shares many properties with FAST-1. We find that this gene, which we call FAST-2, is able to mediate transcriptional activation by TGF-β. FAST-2 also interacts directly with Smad2, a cytoplasmic protein which is translocated to the nucleus in response to TGF- β, and forms a multimeric complex with Smad2 and Smad4 on the activin response element, a high-affinity binding site for FAST-1. Analysis of the sequences of FAST-1 and FAST-2 reveals substantial protein sequence divergence compared to known vertebrate orthologs in the WH family. This suggests that FAST-2 represents a new WH gene related to FAST-1, which functions to mediate TGF-β signals in mammals. We have also examined the structure of the FAST-2 gene and find that it overlaps with a kinesin motor protein gene. The genes are transcribed in opposite orientations, and their transcripts overlap in the 3' untranslated region. |
| Keywords: | signal transduction; sequence analysis; dna-binding proteins; nonhuman; forkhead transcription factors; animals; mice; gene expression; smad2 protein; smad3 protein; transforming growth factor beta; transcription initiation; transcription factor; cos cells; transcription factors; molecular cloning; cloning, molecular; transcription regulation; amino acid sequence; molecular sequence data; nucleotide sequence; base sequence; trans-activators; mammals; luciferases; protein determination; dna, complementary; xenopus; activin; activins; priority journal; article; inhibins; genes, overlapping |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 19 |
| Issue: | 1 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1999-01-01 |
| Start Page: | 424 |
| End Page: | 430 |
| Language: | English |
| PUBMED: | 9858566 |
| PROVIDER: | scopus |
| PMCID: | PMC83900 |
| DOI: | 10.1128/MCB.19.1.424 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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