Cellular basis of B cell clonal populations in old mice Journal Article
| Authors: | LeMaoult, J.; Manavalan, J. S.; Dyall, R.; Szabo, P.; Nikolic-Zugic, J.; Weksler, M. E. |
| Article Title: | Cellular basis of B cell clonal populations in old mice |
| Abstract: | Previous studies from this laboratory have shown that >85% of old mice have stable B cell clonal populations detectable by Ig heavy chain complementary-determining region 3 mRNA size analysis and confirmed by sequence analysis. B cells from the same clone are frequently detected in several lymphoid compartments of the same mouse. We now report the phenotype of all ten stable B cell clonal populations detected in five 20-month-old C57BL/6 mice. These clonal B cells appear to develop in the periphery, and nine of the ten B cell clonal populations expressed the CD5 cell surface marker. Stable B cell expansions may be dominated by cells at two stages of differentiation. Some B cell populations were detected with DNA as well as RNA and represent large clonal populations of B cells, detectable in several lymphoid compartments. These populations are found predominantly in B cell populations expressing CD45R/B220 and the mRNA coding for the membrane-bound form of the μ Ig heavy chain, which suggests a predominance of B lymphocytes in these populations. In other cases, smaller clonal populations were detected only in splenic RNA samples. These clonal populations were found predominantly among CD45R/B220- B cells and did not express the membrane- bound form of the μ Ig heavy chain. We offer the hypothesis that the B cell clonal populations present in old mice may be precursors of the two types of B cell neoplasms which are dominated by CD5+ B cells (B cell chronic lymphocytic leukemia) or plasma cells (multiple myeloma). |
| Keywords: | controlled study; nonhuman; animal cell; mouse; phenotype; animals; mice; bone marrow cells; multiple myeloma; bone marrow; spleen; cell population; mice, inbred c57bl; b lymphocyte; cell lineage; stem cell; immunoglobulin heavy chain; messenger rna; rna, messenger; lymph node; lymphocyte clone; organ specificity; stem cells; aging; chronic lymphatic leukemia; cd5 antigen; cell aging; clone cells; antigens, cd45; receptors, antigen, b-cell; peritoneal cavity; immunoglobulin mu-chains; lymphocyte surface marker; female; priority journal; article; b-lymphocyte subsets |
| Journal Title: | Journal of Immunology |
| Volume: | 162 |
| Issue: | 11 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1999-06-01 |
| Start Page: | 6384 |
| End Page: | 6391 |
| Language: | English |
| PUBMED: | 10352251 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
