Cellular basis of B cell clonal populations in old mice Journal Article


Authors: LeMaoult, J.; Manavalan, J. S.; Dyall, R.; Szabo, P.; Nikolic-Zugic, J.; Weksler, M. E.
Article Title: Cellular basis of B cell clonal populations in old mice
Abstract: Previous studies from this laboratory have shown that >85% of old mice have stable B cell clonal populations detectable by Ig heavy chain complementary-determining region 3 mRNA size analysis and confirmed by sequence analysis. B cells from the same clone are frequently detected in several lymphoid compartments of the same mouse. We now report the phenotype of all ten stable B cell clonal populations detected in five 20-month-old C57BL/6 mice. These clonal B cells appear to develop in the periphery, and nine of the ten B cell clonal populations expressed the CD5 cell surface marker. Stable B cell expansions may be dominated by cells at two stages of differentiation. Some B cell populations were detected with DNA as well as RNA and represent large clonal populations of B cells, detectable in several lymphoid compartments. These populations are found predominantly in B cell populations expressing CD45R/B220 and the mRNA coding for the membrane-bound form of the μ Ig heavy chain, which suggests a predominance of B lymphocytes in these populations. In other cases, smaller clonal populations were detected only in splenic RNA samples. These clonal populations were found predominantly among CD45R/B220- B cells and did not express the membrane- bound form of the μ Ig heavy chain. We offer the hypothesis that the B cell clonal populations present in old mice may be precursors of the two types of B cell neoplasms which are dominated by CD5+ B cells (B cell chronic lymphocytic leukemia) or plasma cells (multiple myeloma).
Keywords: controlled study; nonhuman; animal cell; mouse; phenotype; animals; mice; bone marrow cells; multiple myeloma; bone marrow; spleen; cell population; mice, inbred c57bl; b lymphocyte; cell lineage; stem cell; immunoglobulin heavy chain; messenger rna; rna, messenger; lymph node; lymphocyte clone; organ specificity; stem cells; aging; chronic lymphatic leukemia; cd5 antigen; cell aging; clone cells; antigens, cd45; receptors, antigen, b-cell; peritoneal cavity; immunoglobulin mu-chains; lymphocyte surface marker; female; priority journal; article; b-lymphocyte subsets
Journal Title: Journal of Immunology
Volume: 162
Issue: 11
ISSN: 0022-1767
Publisher: The American Association of Immunologists, Inc  
Date Published: 1999-06-01
Start Page: 6384
End Page: 6391
Language: English
PUBMED: 10352251
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 16 August 2016 -- Source: Scopus
Citation Impact
MSK Authors
  1. Rubendra Dyall
    33 Dyall