Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids Journal Article
| Authors: | Makhija, S.; Taylor, D. D.; Gibb, R. K.; Gerçel-Taylor, Ç |
| Article Title: | Taxol-induced Bcl-2 phosphorylation in ovarian cancer cell monolayer and spheroids |
| Abstract: | Expression of Bcl-2, Bax, p53 and induction of apoptosis were studied in cisplatin or Taxol treated monolayer and spheroid cultures of ovarian cancer cell lines (SKOV-3, UL-1, UL-3C). While cisplatin (15-75 μg/ml) induced apoptosis in monolayer and spheroid cultures, Taxol (100-800 nM) induced fragmentation in monolayers only. Cisplatin induced up to 5-fold DNA fragmentation in monolayers, while 3-fold (UL-3C, SKOV-3), and 1.5-fold (UL-1) in spheroids. Taxol treatment of monolayers resulted in the characteristic phosphorylation of Bcl-2, which was not demonstrated in spheroid cultures. Bax expression was reduced in spheroids following cisplatin or Taxol treatment, while p53 levels remained unchanged. |
| Keywords: | oncoprotein; proto-oncogene proteins; cisplatin; antineoplastic agents; paclitaxel; antineoplastic agent; ovarian cancer; ovarian neoplasms; metabolism; protein bcl 2; apoptosis; antineoplastic agents, phytogenic; drug effect; tumor cells, cultured; phosphorylation; protein p53; biosynthesis; cell culture; ovary tumor; tumor suppressor protein p53; proto-oncogene proteins c-bcl-2; taxol; bcl-2; protein bax; spheroids, cellular; bcl-2-associated x protein; multicellular spheroid; spheroids; humans; human; female; article; bax protein, human |
| Journal Title: | International Journal of Oncology |
| Volume: | 14 |
| Issue: | 3 |
| ISSN: | 1019-6439 |
| Publisher: | Spandidos Publications |
| Date Published: | 1999-03-01 |
| Start Page: | 515 |
| End Page: | 521 |
| Language: | English |
| PUBMED: | 10024685 |
| PROVIDER: | scopus |
| DOI: | 10.3892/ijo.14.3.515 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
