XRCC3 promotes homology-directed repair of DNA damage in mammalian cells Journal Article
| Authors: | Pierce, A. J.; Johnson, R. D.; Thompson, L. H.; Jasin, M. |
| Article Title: | XRCC3 promotes homology-directed repair of DNA damage in mammalian cells |
| Abstract: | Homology-directed repair of DNA damage has recently emerged as a major mechanism for the maintenance of genomic integrity in mammalian cells. The highly conserved strand transferase, Rad51, is expected to be critical for this process. XRCC3 possesses a limited sequence similarity to Rad51 and interacts with it. Using a novel fluorescence-based assay, we demonstrate here that error-free homology-directed repair of DNA double-strand breaks is decreased 25-fold in an XRCC3-deficient hamster cell line and can be restored to wild-type levels through XRCC3 expression. These results establish that XRCC3-mediated homologous recombination can reverse DNA damage that would otherwise be mutagenic or lethal. |
| Keywords: | protein expression; dna-binding proteins; nonhuman; animals; dna damage; homologous recombination; dna repair; gene expression; fluorescence; cell line; luminescent proteins; dna strand breakage; gene conversion; double stranded dna; genetic recombination; recombination, genetic; recombinant proteins; green fluorescent proteins; sequence homology; double-strand break repair; mammal cell; rad51 recombinase; cricetinae; genetic complementation test; transferase; mammalian cells; xrcc3; humans; priority journal; article; rad51-related proteins |
| Journal Title: | Genes and Development |
| Volume: | 13 |
| Issue: | 20 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 1999-10-15 |
| Start Page: | 2633 |
| End Page: | 2638 |
| Language: | English |
| DOI: | 10.1101/gad.13.20.2633 |
| PUBMED: | 10541549 |
| PROVIDER: | scopus |
| PMCID: | PMC317094 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work