Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation Journal Article

Authors: Zhang, Y.; Ng, H. H.; Erdjument-Bromage, H.; Tempst, P.; Bird, A.; Reinberg, D.
Article Title: Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation
Abstract: ATP-dependent nucleosome remodeling and core histone acetylation and deacetylation represent mechanisms to alter nucleosome structure. NuRD is a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. The histone deacetylases HDAC1 and HDAC2 and the histone binding proteins RbAp48 and RbAp46 form a core complex shared between NuRD and Sin3- histone deacetylase complexes. The histone deacetylase activity of the core complex is severely compromised. A novel polypeptide highly related to the metastasis-associated protein 1, MTA2, and the methyl-CpG-binding domain- containing protein, MBD3, were found to be subunits of the NuRD complex. MTA2 modulates the enzymatic activity of the histone deacetylase core complex. MBD3 mediates the association of MTA2 with the core histone deacetylase complex. MBD3 does not directly bind methylated DNA but is highly related to MBD2, a polypeptide that binds to methylated DNA and has been reported to possess demethylase activity. MBD2 interacts with the NuRD complex and directs the complex to methylated DNA. NuRD may provide a means of gene silencing by DNA methylation.
Keywords: unclassified drug; dna-binding proteins; protein dna binding; protein protein interaction; protein binding; hela cells; dna methylation; transcription factors; nuclear proteins; cloning, molecular; dna; amino acid sequence; molecular sequence data; sequence homology, amino acid; recombinant fusion proteins; nucleotide sequence; carrier proteins; autoantigens; base sequence; gene silencing; protein subunit; multienzyme complexes; repressor proteins; adenosine triphosphatases; histone deacetylases; dna helicases; histone deacetylase; nucleosomes; humans; priority journal; article; histone deacetylase complex; nucleosome remodeling; metastasis associated protein 1; methyl cpg binding domain containing protein
Journal Title: Genes and Development
Volume: 13
Issue: 15
ISSN: 0890-9369
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 1999-08-01
Start Page: 1924
End Page: 1935
Language: English
PUBMED: 10444591
PROVIDER: scopus
PMCID: PMC316920
DOI: 10.1101/gad.13.15.1924
Notes: Article -- Export Date: 16 August 2016 -- Source: Scopus
Citation Impact
MSK Authors
  1. Paul J Tempst
    323 Tempst