Organization and structure of the mouse γ-glutamyl hydrolase gene and the functional identification of its promoter Journal Article

  

Authors:	Esaki, T.; Masumoto, N.; Hayes, P.; Chen, J.; Sirotnak, F. M.
Article Title:	Organization and structure of the mouse γ-glutamyl hydrolase gene and the functional identification of its promoter
Abstract:	The organization and structure of the murine GH gene encoding c-glutamyl hydrolase were determined. The murine GH gene spans 24 kb and was found to be distributed in nine exons. The intron/exon coding junctions delineated conformed to the GT-AG rule. The 5' UTR and 3' UTR along with some coding sequences were incorporated in exons 1 and 9, respectively, whereas exons 2-8 incorporated only coding sequence. A relatively GC-rich region of the genome 5' of exon 1 was distinctly promoter-like and encoded a number of putative cis-acting elements, including six Sp1 sites known to be involved in the regulation of transcription but no TATA sequence motif. Primer extension analysis of this region with mouse liver and S180 cell mRNA revealed several tsps within the region encompassing the Sp1 sites. Functional analysis of this 5* upstream region of sequence was carried out by inserting it into the pGL3 reporter gene vector for transfection into NIH3T3 cells. The transcription of the luciferase gene that resulted in these cells established the identity of this region as an active promoter for the mouse GH gene. (C) 1999 Elsevier Science B.V. All rights reserved.
Keywords:	promoter region; exons; nonhuman; animal cell; mouse; animals; mice; animal tissue; transcription, genetic; animalia; gene expression regulation; cloning, molecular; molecular sequence data; nucleotide sequence; murinae; reporter gene; base sequence; gene structure; dna, complementary; promoter; gamma-glutamyl hydrolase; restriction mapping; promoter regions (genetics); gamma glutamyl hydrolase; priority journal; article; glutamyl hydrolase gene
Journal Title:	Gene
Volume:	234
Issue:	1
ISSN:	0378-1119
Publisher:	Elsevier Science, Inc.
Date Published:	1999-06-24
Start Page:	93
End Page:	100
Language:	English
DOI:	10.1016/s0378-1119(99)00174-2
PUBMED:	10393243
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033010145&partnerID=40&md5=d4bf32b347ad12892ec305ade0973a68
Notes:	Article -- Export Date: 16 August 2016 -- Source: Scopus

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