Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins Journal Article
| Authors: | Hittelman, A. B.; Burakov, D.; Iñiguez-Lluhí, J. A.; Freedman, L. P.; Garabedian, M. J. |
| Article Title: | Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins |
| Abstract: | The hormone-activated glucocorticoid receptor (GR), through its N- and C-terminal transcriptional activation functions AF-1 and AF-2, controls the transcription of target genes presumably through interaction(s) with transcriptional regulatory factors. Utilizing a modified yeast two-hybrid approach, we have identified the tumor susceptibility gene 101 (TSG101) and the vitamin D receptor-interacting protein 150 (DRIP150) as proteins that interact specifically with a functional GR AF-1 surface. In yeast and mammalian cells, TSG101 represses whereas DRIP150 enhances GR AF-1-mediated transactivation. Thus, GR AF-1 is capable of recruiting both positive and negative regulatory factors that differentially regulate GR transcriptional enhancement. In addition, we show that another member of the DRIP complex, DRIP205, interacts with the GR ligand binding domain in a hormone-dependent manner and facilitates GR transactivation in concert with DRIP150. These results suggest that DRIP150 and DRIP205 functionally link GR AF-1 and AF-2, and represent important mediators of GR transcriptional enhancement. |
| Keywords: | controlled study; human cell; dna-binding proteins; nonhuman; protein domain; animal cell; mammalia; animals; protein protein interaction; transcription initiation; cell line; protein binding; transcription factor; hela cells; animalia; transcription factors; nuclear proteins; gene expression regulation; in situ hybridization; transcription regulation; transactivation; binding protein; trans-activators; yeast; glucocorticoid receptor; glucocorticoids; receptors, glucocorticoid; mammal cell; two-hybrid system techniques; trans-activation (genetics); tsg101; humans; human; priority journal; article; drip coactivator complex |
| Journal Title: | EMBO Journal |
| Volume: | 18 |
| Issue: | 19 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1999-10-01 |
| Start Page: | 5380 |
| End Page: | 5388 |
| Language: | English |
| DOI: | 10.1093/emboj/18.19.5380 |
| PUBMED: | 10508170 |
| PROVIDER: | scopus |
| PMCID: | PMC1171607 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
