Intradermal injection of granulocyte-macrophage colony-stimulating factor (GM-CSF) in patients with metastatic melanoma recruits dendritic cells Journal Article
| Authors: | Nasi, M. L.; Lieberman, P.; Busam, K. J.; Prieto, V.; Panageas, K. S.; Lewis, J. J.; Houghton, A. N.; Chapman, P. B. |
| Article Title: | Intradermal injection of granulocyte-macrophage colony-stimulating factor (GM-CSF) in patients with metastatic melanoma recruits dendritic cells |
| Abstract: | Dendritic cells (DCs) are the main antigen-presenting cells in the skin. We hypothesized that intradermal (i.d.) injection of granulocyte-macrophage colony-stimulating factor (GM-CSF) would recruit DCs into melanoma skin metastases and enhance autologous melanoma antigen presentation to host T cells. Sixteen patients with cutaneous or subcutaneous melanoma metastases were treated with GM-CSF injected i.d. into a single dermal metastasis and into a normal skin site for 10 consecutive days at one of four dose levels (10, 20, 40, or 80 μg/injection). Pretreatment and post-treatment skin and tumor biopsies were stained for a panel of T-cell, B-cell, macrophage, and DC immunohistochemical markers. Positive cells were quantitated in a blinded fashion. There was a significant increase in the number of DCs (HLA-DR+, S100+, factor XIIIa+) and CD45RO+ T cells in the skin and in the tumors injected with GM-CSF at all dose levels. Uninjected control tumors showed no increase in HLA-DR+ cells or T-cell infiltrate, but did show an increase in S100+ and factor XIIIa+ cells, suggesting a non-DC population. ID GM-CSF administered in this manner recruited DCs into melanoma tumors and normal skin. Although no antitumor effects were seen, this represents a potential method of preparing skin sites for vaccine delivery. |
| Keywords: | immunohistochemistry; clinical article; controlled study; human tissue; clinical trial; t lymphocyte; melanoma; controlled clinical trial; skin neoplasms; dendritic cell; granulocyte macrophage colony stimulating factor; tumor biopsy; b lymphocyte; antigen presentation; dendritic cells; antigens, neoplasm; hla dr antigen; protein s 100; injections, intradermal; macrophage; growth factor; skin metastasis; antigen presenting cell; cd45 antigen; vaccine; intradermal drug administration; hypothesis; immunity, cellular; t cell; granulocyte-macrophage colony-stimulating factor; blood clotting factor 13a; humans; human; male; female; priority journal; article |
| Journal Title: | Cytokines Cellular and Molecular Therapy |
| Volume: | 5 |
| Issue: | 3 |
| ISSN: | 1368-4736 |
| Publisher: | Taylor & Francis Group |
| Date Published: | 1999-09-01 |
| Start Page: | 139 |
| End Page: | 144 |
| Language: | English |
| PUBMED: | 10641571 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
