Mesenchymal to epithelial conversion in rat metanephros is induced by LIF Journal Article
| Authors: | Barasch, J.; Yang, J.; Ware, C. B.; Taga, T.; Yoshida, K.; Erdjument-Bromage, H.; Tempst, P.; Parravicini, E.; Malach, S.; Aranoff, T.; Oliver, J. A. |
| Article Title: | Mesenchymal to epithelial conversion in rat metanephros is induced by LIF |
| Abstract: | Inductive signals cause conversion of mesenchyme into epithelia during the formation of many organs. Yet a century of study has not revealed the inducing molecules. Using a standard model of induction, we found that ureteric bud cells secrete factors that convert kidney mesenchyme to epithelia that, remarkably, then form nephrons. Purification and sequencing of one such factor identified it as leukemia inhibitory factor (LIF). LIF acted on epithelial precursors that we identified by the expression of Pax2 and Wnt4. Other IL-6 type cytokines acted like LIF, and deletion of their shared receptor reduced nephron development. In situ, the ureteric bud expressed LIF, and metanephric mesenchyme expressed its receptors. The data suggest that IL-6 cytokines are candidate regulators of mesenchymal to epithelial conversion during kidney development. |
| Keywords: | signal transduction; dna-binding proteins; proto-oncogene proteins; nonhuman; animal cell; animals; mice; cell differentiation; animalia; transcription factors; fibroblast growth factor 2; membrane glycoproteins; recombinant proteins; rat; interleukin-6; epithelium cell; ligands; cytokine receptor gp130; stat3 transcription factor; rats; trans-activators; antigens, cd; epithelium; ureter; nephrons; wnt proteins; mesoderm; mesenchyme; kidney development; pax2 transcription factor; growth inhibitors; leukemia inhibitory factor; embryonic induction; kidney tubules; kidney epithelium; receptors, cytokine; leukemia inhibitory factor receptor alpha subunit; priority journal; article; lymphokines; receptors, osm-lif |
| Journal Title: | Cell |
| Volume: | 99 |
| Issue: | 4 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1999-11-12 |
| Start Page: | 377 |
| End Page: | 386 |
| Language: | English |
| PUBMED: | 10571180 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0092-8674(00)81524-X |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
