Multimodal cancer treatment mediated by a replicating oncolytic virus that delivers the oxazaphosphorine/rat cytochrome P450 2B1 and ganciclovir/herpes simplex virus thymidine kinase gene therapies Journal Article
| Authors: | Aghi, M.; Chou, T. C.; Suling, K.; Breakefield, X. O.; Chiocca, E. A. |
| Article Title: | Multimodal cancer treatment mediated by a replicating oncolytic virus that delivers the oxazaphosphorine/rat cytochrome P450 2B1 and ganciclovir/herpes simplex virus thymidine kinase gene therapies |
| Abstract: | Multimodal therapy is generally more effective than single-agent treatment for cancer. rRp450 is an engineered herpes simplex viral mutant that replicates in and kills tumor cells in a relatively selective fashion. It also expresses, in infected cells, the cyclophosphamide (CPA)-sensitive rat cytochrome P450 2B1 (CYP2B1) and the ganciclovir (GCV)-sensitive herpes simplex virus thymidine kinase (HSV-TK) transgenes. We show that cultured rat 9L and human U87ΔEGFR glioma cells, infected and lysed by rRp450, also exhibit supra-additive sensitivity to both CPA and GCV, as determined by Chou-Talalay synergy analysis. DNA cross-linking, assayed by ethidium bromide fluorescence, was significantly inhibited in the presence of GCV, suggesting that interactions between the CPA/CYP2B1 and GCV/HSV-TK gene therapies occurred at the level of DNA repair. In vivo, regression of 9L s.c. tumor volumes in athymic mice was achieved only by the multimodal treatment allowed by rRp450 viral oncolysis combined with CPA/CYP2B1 and GCV/HSV-TK gene therapies, whereas all other treatment combinations produced only tumor growth retardation. |
| Keywords: | multimodality cancer therapy; nonhuman; antineoplastic agents; combined modality therapy; glioma; animal cell; animals; mice; cell death; cyclophosphamide; drug effect; tumor cells, cultured; mice, nude; rat; gene therapy; oncolytic virus; thymidine kinase; rats; virus replication; tumor growth; herpes simplex virus; ganciclovir; cytochrome p450; virus mutant; growth inhibition; gene transfer techniques; cross linking; viruses; humans; priority journal; article; oxazaphosphorine derivative; cytochrome p-450 cyp2b1 |
| Journal Title: | Cancer Research |
| Volume: | 59 |
| Issue: | 16 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1999-08-15 |
| Start Page: | 3861 |
| End Page: | 3865 |
| Language: | English |
| PUBMED: | 10463570 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
