Constitutive overexpression of cyclin D(1) in human breast epithelial cells does not prevent G(1) arrest induced by deprivation of epidermal growth factor Journal Article

  

Authors:	Chou, J. L.; Fan, Z.; DeBlasio, T.; Koff, A.; Rosen, N.; Mendelsohn, J.
Article Title:	Constitutive overexpression of cyclin D(1) in human breast epithelial cells does not prevent G(1) arrest induced by deprivation of epidermal growth factor
Abstract:	Non-transformed human breast epithelial cell line MCF10A is dependent on exogenous epidermal growth factor (EGF) for continued growth. Complete G1 arrest was rapidly induced following EGF deprivation. The cell cycle arrest was accompanied by increased levels of p27(KIP1), a cyclin-dependent kinase inhibitor, and reduced level of cyclin D1. This was associated with strong inhibition of cyclin-dependent kinase 2 and cyclin D1-associated kinase activities. Introduction of exogenous cyclin D1 into MCF10A (MCF10AD1) cells resulted in an accelerated cell growth rate but did not confer colony-forming capacity. Cell cycle arrest was still achieved in MCF10AD1 cells following EGF deprivation. In the great majority of MCF10AD1 clones, accumulation in G1 phase was accompanied by reduced cyclin D1 and increased p27(KIP1) protein levels. In two clones where cyclin D1 remained unchanged during G1 arrest, it was found that more cyclin D1 protein was bound to p27(KIP1). The data demonstrate that ectopic expression of cyclin D1 alone could nor transform MCF10A cells nor was it sufficient to prevent G1 arrest induced by EGF deprivation.
Keywords:	epidermal growth factor; controlled study; protein expression; human cell; cell cycle proteins; cell cycle; cell division; cell growth; breast; cell line; protein binding; enzyme activity; phosphorylation; antibodies, monoclonal; protein-serine-threonine kinases; protein p27; cyclin-dependent kinase inhibitor p27; tumor suppressor proteins; breast epithelium; epithelial cells; cyclin-dependent kinase inhibitor p21; cyclin d1; cyclin-dependent kinases; cyclins; cyclin dependent kinase; retinoblastoma protein; phosphotransferase; cell cycle g1 phase; g1 phase; cyclin-dependent kinase 2; colony formation; microtubule-associated proteins; G1 arrest; cdc2-cdc28 kinases; humans; human; priority journal; article; egf deprivation
Journal Title:	Breast Cancer Research and Treatment
Volume:	55
Issue:	3
ISSN:	0167-6806
Publisher:	Springer
Date Published:	1999-06-01
Start Page:	267
End Page:	283
Language:	English
DOI:	10.1023/a:1006217413089
PUBMED:	10517171
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032867174&partnerID=40&md5=5f49ab52240c87c66dee2f5039e9ba83
Notes:	Article -- Export Date: 16 August 2016 -- Source: Scopus

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