A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells Journal Article

   


Authors: Quann, E. J.; Liu, X.; Altan-Bonnet, G.; Huse, M.
Article Title: A cascade of protein kinase C isozymes promotes cytoskeletal polarization in T cells
Abstract: Polarization of the T cell microtubule-organizing center (MTOC) toward the antigen-presenting cell (APC) is driven by the accumulation of diacylglycerol (DAG) at the immunological synapse (IS). The mechanisms that couple DAG to the MTOC are not known. By single-cell photoactivation of the T cell antigen receptor (TCR), we found that three distinct isoforms of protein kinase C (PKC) were recruited by DAG to the IS in two steps. PKC-ε and PKC-η accumulated first in a broad region of membrane, whereas PKC-θ arrived later in a smaller zone. Functional experiments indicated that PKC-θ was required for MTOC reorientation and that PKC-ε and PKC-η operated redundantly to promote the recruitment of PKC-θ and subsequent polarization responses. Our results establish a previously uncharacterized role for PKC proteins in T cell polarity. © 2011 Nature America, Inc. All rights reserved.
Keywords: controlled study; nonhuman; protein function; protein localization; t lymphocyte; animal cell; mouse; animal tissue; animal experiment; t lymphocyte receptor; cell membrane; cell polarity; cytoskeleton; antigen presenting cell; diacylglycerol; photoactivation; immunological synapse; protein kinase c epsilon; protein kinase c theta; protein kinase c eta
Journal Title: Nature Immunology
Volume: 12
Issue: 7
ISSN: 1529-2908
Publisher: Nature Publishing Group  
Date Published: 2011-07-01
Start Page: 647
End Page: 654
Language: English
DOI: 10.1038/ni.2033
PROVIDER: scopus
PMCID: PMC3119370
PUBMED: 21602810
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-79959376445&partnerID=40&md5=19f8ce19f161a7299045915f26ae028d
Notes: --- - "Export Date: 17 August 2011" - "CODEN: NIAMC" - "Source: Scopus"
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MSK Authors
  1. Gregoire Altan-Bonnet
    42 Altan-Bonnet
  2. Morgan Huse
    68 Huse
  3. Emily Jean Quann
    6 Quann
  4. Xin Liu
    7 Liu
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