Deep depletion of abundant serum proteins reveals low-abundant proteins as potential biomarkers for human ovarian cancer Journal Article
| Authors: | Lin, B.; White, J. T.; Wu, J.; Lele, S.; Old, L. J.; Hood, L.; Odunsi, K. |
| Article Title: | Deep depletion of abundant serum proteins reveals low-abundant proteins as potential biomarkers for human ovarian cancer |
| Abstract: | Epithelial ovarian cancer (EOC) ranks fifth as a cause of cancer deaths in women. Current diagnostic and monitoring markers have limited reliability for the detection of disease. We have tested the possibility of identifying candidate biomarkers present at low nanogram to picogram levels after removing both the 12 most abundant and 77 moderately abundant proteins from serum samples of EOC patients using antibody affinity columns. We showed that this approach allows the identification of proteins that are expressed at nanogram per liter levels in the serum. Using ICAT/MS/MS analysis, we identified 51 proteins that are differentially expressed by at least twofold. These proteins include leucine-rich α-2-glycoprotein, matrix metalloproteinase-9 (MMP-9), inter-α-trypsin inhibitor heavy chain H1, insulin-like growth factor-binding protein 6, insulin-like growth factor-binding protein 3, isoform 1 of epidermal growth factor receptor, angiopoietin-like protein 3 (ANGPTL3) and phosphatidylcholine-sterol acyltransferase. We confirmed the differential expression of MMP9 and ANGPTL3 in normal and ovarian cancer sera by ELISA assays. Further robust clinical evaluation of the candidate markers identified is necessary. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| Keywords: | protein expression; ovarian cancer; mass spectrometry; protein analysis; protein blood level; ovary cancer; epidermal growth factor receptor; gelatinase b; enzyme linked immunosorbent assay; cancer mortality; angiopoietin-like protein 3; matrix metalloproteinase-9; ms; serum depletion; alpha2 glycoprotein; angiopoietin 2; blood clotting factor 9; complement component c2; connective tissue growth factor; endothelial leukocyte adhesion molecule 1; hemopexin; inter alpha trypsin inhibitor; phosphatidylcholine sterol acyltransferase; pregnancy associated alpha2 glycoprotein; somatomedin binding protein 3; somatomedin binding protein 6; vitronectin; antibody affinity |
| Journal Title: | Proteomics Clinical Applications |
| Volume: | 3 |
| Issue: | 7 |
| ISSN: | 1862-8346 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2009-07-01 |
| Start Page: | 853 |
| End Page: | 861 |
| Language: | English |
| DOI: | 10.1002/prca.200800141 |
| PROVIDER: | scopus |
| PMCID: | PMC2886282 |
| PUBMED: | 20559449 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 6" - "Export Date: 30 November 2010" - "Source: Scopus" |
