RNAi in Xenopus: Look before you leap Journal Article
| Authors: | Flynt, A. S.; Lai, E. C. |
| Article Title: | RNAi in Xenopus: Look before you leap |
| Abstract: | RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiquitous or constitutive. Lund and colleagues (pp. 1121-1131) show that microRNA (miRNA) effector Argonautes (Agos) are limiting and easily saturated during early Xenopus embryogenesis. Moreover, this stage is devoid of slicing capacity. Supplementation of Ago proteins rescued endogenous miRNA activity in the presence of exogenous siRNAs, and, excitingly, ectopic Ago2 could now support RNAi in Xenopus. These observationsmay potentially facilitate RNAi in other recalcitrant model organisms. © 2011 by Cold Spring Harbor Laboratory Press. |
| Keywords: | protein expression; unclassified drug; nonhuman; oocyte; gene; gene expression; embryo; small interfering rna; rna interference; upregulation; fish; oligonucleotide; argonaute protein; argonaute 1 protein; dicer; argonaute 2 protein; xenopus; rnai; sirna; ago2; exportin 5; argonautes; xenopus embryos; protein ago3; protein ago4 |
| Journal Title: | Genes and Development |
| Volume: | 25 |
| Issue: | 11 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2011-06-01 |
| Start Page: | 1105 |
| End Page: | 1108 |
| Language: | English |
| DOI: | 10.1101/gad.2062911 |
| PROVIDER: | scopus |
| PMCID: | PMC3110948 |
| PUBMED: | 21632820 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 17 August 2011" - "CODEN: GEDEE" - "Source: Scopus" |
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