Locked nucleic acid probes (LNA) for enhanced detection of low-level, clinically significant mutations Journal Article
| Authors: | Nafa, K.; Hameed, M.; Arcila, M. E. |
| Article Title: | Locked nucleic acid probes (LNA) for enhanced detection of low-level, clinically significant mutations |
| Abstract: | The detection of clinically significant somatic mutations present at low level in a tissue sample represents a challenge in any laboratory. While several high sensitivity methods are described, the incorporation of these new techniques in a clinical lab may be difficult if the technology is not readily available or requires major changes in the workfl ow of the laboratory. Techniques that are robust and easily adapted to existing laboratory protocols are highly advantageous. In this chapter we describe the use of locked nucleic acid (LNA) probes to modify existing polymerase chain reaction (PCR)-based protocols which can then be sequenced by Sanger sequencing. LNA probes are used to enhance the sensitivity of Sanger sequencing to mutation frequencies below 1%. The method is robust and is easily incorporated for assessment of any sample with low tumor content or low mutant allele burden. |
| Keywords: | assay; polymorphisms; real-time pcr; primers; discrimination; lna; locked nucleic acid probes; lna clamping; lna-pcr |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1392 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Publication Place: | Totowa, NJ |
| Date Published: | 2016-01-01 |
| Start Page: | 71 |
| End Page: | 82 |
| Language: | English |
| ACCESSION: | WOS:000371263300009 |
| PROVIDER: | wos |
| PUBMED: | 26843048 |
| DOI: | 10.1007/978-1-4939-3360-0_8 |
| Notes: | Article; Book Chapter 8 in "Clinical Applications of PCR" (ISBN: 978-1-4939-3358-7) -- Source: Wos |
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