Interaction of extracellular matrix and activin-A in the initiation of follicle growth in the mouse ovary Journal Article

Authors: Oktay, K.; Karlikaya, G.; Akman, O.; Ojakian, G. K.; Oktay, M.
Article Title: Interaction of extracellular matrix and activin-A in the initiation of follicle growth in the mouse ovary
Abstract: The precise mechanism for the initiation of follicle growth and progression through the earliest stages of follicle development remains largely unknown. Activins play a role during early follicle development, and evidence suggests that the extracellular matrix plays a role during later stages of follicular growth. We investigated the role of activin-A and extracellular matrix in follicle growth initiation and early follicular development in the mouse ovary. Ovaries were collected from Ei-day-old mice and cultured for 10 days on polylysine, collagen, or laminin in the presence or absence of recombinant human activin-A. Follicle density, indices of follicle growth initiation (primary:primordial follicle [PY:PD] and primary:total follicle [PY:TF] ratios), ratios of multilayer follicle:total follicle (ML:TF), and follicle growth rates were compared between groups. Follicle densities were significantly higher in the extracellular matrix treatment group compared with the polylysine group (P < 0.01). Also, compared with polylysine, both collagen and laminin significantly increased indices of follicle growth initiation (PY:PD ratio: P < 0.001, odds ratio of 3.3; PY:TF ratio: P < 0.001, odds ratio of 2.5), and these were not altered by activin treatment. In the absence of activin-A, exposure to neither collagen nor laminin had an effect on multilayer follicle development. When activin-A was added, collagen and laminin had opposing effects on multilayer follicle development. Activin-A stimulated multilayer follicle development in the presence of laminin (ML:TF ratio: P = 0.01, odds ratio of 10.8), whereas it suppressed follicle growth in collagen (P = 0.01). Activin-A did not affect the ML:TF ratio in the polylysine-treated groups. These results strongly suggest that extracellular matrix components and activin-A interact with each other, and that they regulate follicle growth initiation and multilayer follicle development.
Keywords: mice; progression; receptor; integrin; differentiation; fibronectin; localization; immunohistochemical; follicular development; activin; follicle; fsh; rat granulosa-cells; stimulating-hormone; folliculogenesis
Journal Title: Biology of Reproduction
Volume: 63
Issue: 2
ISSN: 0006-3363
Publisher: Society for the Study of Reproduction  
Date Published: 2000-08-01
Start Page: 457
End Page: 461
Language: English
ACCESSION: WOS:000088491700015
DOI: 10.1095/biolreprod63.2.457
PUBMED: 10906050
Notes: Article -- Source: Wos
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MSK Authors
  1. Maja H Oktay
    4 Oktay