Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b Journal Article

  


Authors: Aktuganov, G.; Jokela, J.; Kivelä, H.; Khalikova, E.; Melentjev, A.; Galimzianova, N.; Kuzmina, L.; Kouvonen, P.; Himanen, J. P.; Susi, P.; Korpela, T.
Article Title: Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b
Abstract: Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water-methanol extraction followed by a chromatography on a C18-support. The analysis with LC-MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C15, fengycin/plipastatin A-C16 together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b. © 2014 Elsevier B.V.
Keywords: unclassified drug; nonhuman; mass spectrometry; protein analysis; bacteria (microorganisms); fatty acids; amino acids; liquid chromatography; thin layer chromatography; fungi; fungus growth; protein isolation; antifungal activity; amino acid analysis; purification; amino acid composition; lc-ms; acetone; lipopeptide; article; gel permeation chromatography; column chromatography; antifungal compounds; biocontrol; cyclic lipopeptides; microbial antagonism; paenibacillus ehimensis; 2,4 diaminobutyric acid; bacillomycin l; hydroxy fatty acid; paenibacillus; cyclic lipopeptide
Journal Title: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume: 973
ISSN: 1570-0232
Publisher: Elsevier Science BV  
Date Published: 2014-12-15
Start Page: 9
End Page: 16
Language: English
DOI: 10.1016/j.jchromb.2014.09.042
PROVIDER: scopus
PUBMED: 25464089
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84949114861&partnerID=40&md5=3d7c2396052565d80542296ba483a986
Notes: Article -- 1 -- Export Date: 7 January 2016 -- 9 -- Source: Scopus
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  1. Juha P Himanen
    50 Himanen
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