Imaging transgene expression with radionuclide imaging technologies Journal Article

   


Authors: Gambhir, S. S.; Herschman, H. R.; Cherry, S. R.; Barrio, J. R.; Satyamurthy, N.; Toyokuni, T.; Phelps, M. E.; Larson, S. M.; Balatoni, J.; Finn, R.; Sadelain, M.; Tjuvajev, J.; Blasberg, R.
Article Title: Imaging transgene expression with radionuclide imaging technologies
Abstract: A variety of imaging technologies are being investigated as tools for studying gene expression in living subjects. Noninvasive, repetitive and quantitative imaging of gene expression will help both to facilitate human gene therapy trials and to allow for the study of animal models of molecular and cellular therapy. Radionuclide approaches using single photon emission computed tomography (SPECT) and positron emission tomography (PET) are the most mature of the current imaging technologies and offer many advantages for imaging gene expression compared to optical and magnetic resonance imaging (MRI)-based approaches. These advantages include relatively high sensitivity, full quantitative capability (for PET), and the ability to extend small animal assays directly into clinical human applications. We describe a PET scanner (microPET) designed specifically for studies of small animals. We review 'marker/reporter gene' imaging approaches using the herpes simplex type 1 virus thymidine kinase (HSV1-tk) and the dopamine type 2 receptor (D2R) genes. We describe and contrast several radiolabeled probes that can be used with the HSV1-tk reporter gene both for SPECT and for PET imaging. We also describe the advantages/disadvantages of each of the assays developed and discuss future animal and human applications.
Keywords: clinical trial; review; nonhuman; neurotoxicity; nuclear magnetic resonance imaging; positron emission tomography; animals; mice; liver toxicity; gene expression; models, biological; aciclovir; gene therapy; transgene; scintiscanning; reporter gene; imaging; fialuridine; thymidine kinase; herpesvirus 1, human; radioisotope; single photon emission computer tomography; pet; herpes simplex virus 1; herpes simplex virus; ganciclovir; radionuclide imaging; transgenes; marker gene; tomography, emission-computed; micropet; spect; penciclovir; whole body autoradiography; dopamine 2 receptor; idoxuridine; receptors, dopamine d2; humans; human; priority journal
Journal Title: NeoPlasia
Volume: 2
Issue: 1-2
ISSN: 1522-8002
Publisher: Elsevier Science Inc.  
Date Published: 2000-01-01
Start Page: 118
End Page: 138
Language: English
PUBMED: 10933072
PROVIDER: scopus
PMCID: PMC1550287
DOI: 10.1038/sj.neo.7900083
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0034048929&partnerID=40&md5=0a79a046f168de856a9fecc41da11baa
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Juri Gelovani
    125 Gelovani
  2. Ronald G Blasberg
    272 Blasberg
  3. Julius A Balatoni
    61 Balatoni
  4. Ronald D Finn
    279 Finn
  5. Michel W J Sadelain
    583 Sadelain
  6. Steven M Larson
    959 Larson
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