Cerebrospinal fluid-derived circulating tumour DNA better represents the genomic alterations of brain tumours than plasma Journal Article


Authors: De Mattos-Arruda, L.; Mayor, R.; Ng, C. K. Y.; Weigelt, B.; Martínez-Ricarte, F.; Torrejon, D.; Oliveira, M.; Arias, A.; Raventos, C.; Tang, J.; Guerini Rocco, E.; Martínez-Saéz, E.; Lois, S.; Marín, O.; De La Cruz, X.; Piscuoglio, S.; Towers, R.; Vivancos, A.; Peg, V.; Cajal, S. R. Y.; Carles, J.; Rodon, J.; González-Cao, M.; Tabernero, J.; Felip, E.; Sahuquillo, J.; Berger, M. F.; Cortes, J.; Reis-Filho, J. S.; Seoane, J.
Article Title: Cerebrospinal fluid-derived circulating tumour DNA better represents the genomic alterations of brain tumours than plasma
Abstract: Cell-free circulating tumour DNA (ctDNA) in plasma has been shown to be informative of the genomic alterations present in tumours and has been used to monitor tumour progression and response to treatments. However, patients with brain tumours do not present with or present with low amounts of ctDNA in plasma precluding the genomic characterization of brain cancer through plasma ctDNA. Here we show that ctDNA derived from central nervous system tumours is more abundantly present in the cerebrospinal fluid (CSF) than in plasma. Massively parallel sequencing of CSF ctDNA more comprehensively characterizes the genomic alterations of brain tumours than plasma, allowing the identification of actionable brain tumour somatic mutations. We show that CSF ctDNA levels longitudinally fluctuate in time and follow the changes in brain tumour burden providing biomarkers to monitor brain malignancies. Moreover, CSF ctDNA is shown to facilitate and complement the diagnosis of leptomeningeal carcinomatosis. © 2015 Macmillan Publishers Limited.
Keywords: controlled study; human tissue; unclassified drug; gene mutation; somatic mutation; brain tumor; sensitivity analysis; dna; cerebrospinal fluid; brain metastasis; breast metastasis; concentration (parameters); carcinomatous meningitis; plasma; human; female; article; cell free circulating tumour dna
Journal Title: Nature Communications
Volume: 6
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2015-11-01
Start Page: 8839
Language: English
DOI: 10.1038/ncomms9839
PROVIDER: scopus
PUBMED: 26554728
PMCID: PMC5426516
DOI/URL:
Notes: Export Date: 2 December 2015 -- Source: Scopus
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  1. Michael Forman Berger
    768 Berger
  2. Russell Towers
    13 Towers
  3. Britta Weigelt
    640 Weigelt
  4. Kiu Yan Charlotte Ng
    155 Ng
  5. Jiabin   Tang
    6 Tang