Dual-modality optical/PET imaging of PARP1 in glioblastoma Journal Article
| Authors: | Carlucci, G.; Carney, B.; Brand, C.; Kossatz, S.; Irwin, C. P.; Carlin, S. D.; Keliher, E. J.; Weber, W.; Reiner, T. |
| Article Title: | Dual-modality optical/PET imaging of PARP1 in glioblastoma |
| Abstract: | Purpose: The current study presents [18F]PARPi-FL as a bimodal fluorescent/positron emission tomography (PET) agent for PARP1 imaging. Procedures: [18F]PARPi-FL was obtained by 19F/18F isotopic exchange and PET experiments, biodistribution studies, surface fluorescence imaging, and autoradiography carried out in a U87 MG glioblastoma mouse model. Results: [18F]PARPi-FL showed high tumor uptake in vivo and ex vivo in small xenografts (< 2 mm) with both PET and optical imaging technologies. Uptake of [18F]PARPi-FL in blocked U87 MG tumors was reduced by 84 % (0.12 ± 0.02 %injected dose/gram (%ID/g)), showing high specificity of the binding. PET imaging showed accumulation in the tumor (1 h p.i.), which was confirmed by ex vivo phosphor autoradiography. Conclusions: The fluorescent component of [18F]PARPi-FL enables cellular resolution optical imaging, while the radiolabeled component of [18F]PARPi-FL allows whole-body deep-tissue imaging of malignant growth. © 2015, World Molecular Imaging Society. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; nonhuman; positron emission tomography; mouse; animal tissue; fluorescence; animal experiment; animal model; in vivo study; in vitro study; molecular imaging; drug distribution; whole body imaging; glioblastoma; imaging; tracer; ex vivo study; pet; autoradiography; fluorescence imaging; olaparib; radiochemistry; reaction time; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1; multimodal imaging; reversed phase high performance liquid chromatography; fluorination; plasma half life; multimodality; priority journal; article; parp1; u87 mg; parpi fl f 18 |
| Journal Title: | Molecular Imaging and Biology |
| Volume: | 17 |
| Issue: | 6 |
| ISSN: | 1536-1632 |
| Publisher: | Springer |
| Date Published: | 2015-12-01 |
| Start Page: | 848 |
| End Page: | 855 |
| Language: | English |
| DOI: | 10.1007/s11307-015-0858-0 |
| PROVIDER: | scopus |
| PMCID: | PMC4609241 |
| PUBMED: | 25895168 |
| DOI/URL: | |
| Notes: | Export Date: 2 December 2015 -- Source: Scopus |
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