Genomic DNA transposition induced by human PGBD5 Journal Article
| Authors: | Henssen, A. G.; Henaff, E.; Jiang, E.; Eisenberg, A. R.; Carson, J. R.; Villasante, C. M.; Ray, M.; Still, E.; Burns, M.; Gandara, J.; Feschotte, C.; Mason, C. E.; Kentsis, A. |
| Article Title: | Genomic DNA transposition induced by human PGBD5 |
| Abstract: | Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containing inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic remodeling contributes to its biological function. © Henssen et al. |
| Keywords: | unclassified drug; nonhuman; polymerase chain reaction; reverse transcription polymerase chain reaction; gene expression; genetic transduction; amino acid sequence; genetic transfection; sequence alignment; western blotting; dna sequence; high performance liquid chromatography; mutagenesis; genomic dna; bacterium contamination; colony formation; neomycin; peptides and proteins; dna integration; dna transposition; mycoplasma; human; article; hek293 cell line; piggybac transposable element derived 5; inverted terminal repeat |
| Journal Title: | eLife |
| Volume: | 4 |
| ISSN: | 2050-084X |
| Publisher: | eLife Sciences Publications Ltd. |
| Date Published: | 2015-09-25 |
| Start Page: | e10565 |
| Language: | English |
| DOI: | 10.7554/eLife.10565 |
| PROVIDER: | scopus |
| PMCID: | PMC4625184 |
| PUBMED: | 26406119 |
| DOI/URL: | |
| Notes: | Export Date: 2 December 2015 -- Source: Scopus |
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