Genomic DNA transposition induced by human PGBD5 Journal Article

Authors: Henssen, A. G.; Henaff, E.; Jiang, E.; Eisenberg, A. R.; Carson, J. R.; Villasante, C. M.; Ray, M.; Still, E.; Burns, M.; Gandara, J.; Feschotte, C.; Mason, C. E.; Kentsis, A.
Article Title: Genomic DNA transposition induced by human PGBD5
Abstract: Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containing inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic remodeling contributes to its biological function. © Henssen et al.
Keywords: unclassified drug; nonhuman; polymerase chain reaction; reverse transcription polymerase chain reaction; gene expression; genetic transduction; amino acid sequence; genetic transfection; sequence alignment; western blotting; dna sequence; high performance liquid chromatography; mutagenesis; genomic dna; bacterium contamination; colony formation; neomycin; peptides and proteins; dna integration; dna transposition; mycoplasma; human; article; hek293 cell line; piggybac transposable element derived 5; inverted terminal repeat
Journal Title: eLife
Volume: 4
ISSN: 2050-084X
Publisher: eLife Sciences Publications Ltd.  
Date Published: 2015-09-25
Start Page: e10565
Language: English
DOI: 10.7554/eLife.10565
PROVIDER: scopus
PMCID: PMC4625184
PUBMED: 26406119
Notes: Export Date: 2 December 2015 -- Source: Scopus
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MSK Authors
  1. Alex   Kentsis
    52 Kentsis
  2. Eileen   Jiang
    5 Jiang
  3. Julianne Rose Carson
    2 Carson
  4. Mondira   Ray
    1 Ray
  5. Eric Ryan Still
    9 Still